Immediate remote ischemic postconditioning after hypoxia ischemia in piglets protects cerebral white matter but not grey matter

Ezzati, M, Bainbridge, A, Kawano, G, Oliver-Taylor, A, Rocha-Ferreira, E, Alonso-Alconada, D, Fierens, I, Rostami, J, Hassell, J, Tachtsidis, I, Gressens, P, Hristova, M, Bennett, K, Lebon, S, Fleiss, B, Yellon, D, Hausenloy, D, Golay, X and Robertson, N 2016, 'Immediate remote ischemic postconditioning after hypoxia ischemia in piglets protects cerebral white matter but not grey matter', Journal of Cerebral Blood Flow and Metabolism, vol. 36, no. 8, pp. 1396-1411.


Document type: Journal Article
Collection: Journal Articles

Title Immediate remote ischemic postconditioning after hypoxia ischemia in piglets protects cerebral white matter but not grey matter
Author(s) Ezzati, M
Bainbridge, A
Kawano, G
Oliver-Taylor, A
Rocha-Ferreira, E
Alonso-Alconada, D
Fierens, I
Rostami, J
Hassell, J
Tachtsidis, I
Gressens, P
Hristova, M
Bennett, K
Lebon, S
Fleiss, B
Yellon, D
Hausenloy, D
Golay, X
Robertson, N
Year 2016
Journal name Journal of Cerebral Blood Flow and Metabolism
Volume number 36
Issue number 8
Start page 1396
End page 1411
Total pages 16
Publisher Sage Publications Ltd.
Abstract Remote ischemic postconditioning (RIPostC) is a promising therapeutic intervention whereby brief episodes of ischemia/reperfusion of one organ (limb) mitigate damage in another organ (brain) that has experienced severe hypoxia-ischemia. Our aim was to assess whether RIPostC is protective following cerebral hypoxia-ischemia in a piglet model of neonatal encephalopathy (NE) using magnetic resonance spectroscopy (MRS) biomarkers and immunohistochemistry. After hypoxia-ischemia (HI), 16 Large White female newborn piglets were randomized to: (i) no intervention (n = 8); (ii) RIPostC - with four, 10-min cycles of bilateral lower limb ischemia/reperfusion immediately after HI (n = 8). RIPostC reduced the hypoxic-ischemic-induced increase in white matter proton MRS lactate/N acetyl aspartate (p = 0.005) and increased whole brain phosphorus-31 MRS ATP (p = 0.039) over the 48 h after HI. Cell death was reduced with RIPostC in the periventricular white matter (p = 0.03), internal capsule (p = 0.002) and corpus callosum (p = 0.021); there was reduced microglial activation in corpus callosum (p = 0.001) and more surviving oligodendrocytes in corpus callosum (p = 0.029) and periventricular white matter (p = 0.001). Changes in gene expression were detected in the white matter at 48 h, including K ATP channel and endothelin A receptor. Immediate RIPostC is a potentially safe and promising brain protective therapy for babies with NE with protection in white but not grey matter.
Subject Innate Immunity
Neurology and Neuromuscular Diseases
Paediatrics
Keyword(s) Birth asphyxia
hypoxia-ischemia
neonatal encephalopathy
neuroprotection
remote ischemic postconditioning
DOI - identifier 10.1177/0271678X15608862
Copyright notice © The Author(s) 2015.
ISSN 0271-678X
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