Role of microglia in a mouse model of paediatric traumatic brain injury

Chhor, V, Moretti, R, Le Charpentier, T, Sigaut, S, Lebon, S, Schwendimann, L, Ore, M, Zuiani, C, Milan, V, Josserand, J, Vontell, R, Pansiot, J, Degos, V, Ikonomidou, C, Titomanlio, L, Hagberg, H, Gressens, P and Fleiss, B 2017, 'Role of microglia in a mouse model of paediatric traumatic brain injury', Brain, Behavior, and Immunity, vol. 63, pp. 197-209.


Document type: Journal Article
Collection: Journal Articles

Title Role of microglia in a mouse model of paediatric traumatic brain injury
Author(s) Chhor, V
Moretti, R
Le Charpentier, T
Sigaut, S
Lebon, S
Schwendimann, L
Ore, M
Zuiani, C
Milan, V
Josserand, J
Vontell, R
Pansiot, J
Degos, V
Ikonomidou, C
Titomanlio, L
Hagberg, H
Gressens, P
Fleiss, B
Year 2017
Journal name Brain, Behavior, and Immunity
Volume number 63
Start page 197
End page 209
Total pages 13
Publisher Academic Press
Abstract The cognitive and behavioural deficits caused by traumatic brain injury (TBI) to the immature brain are more severe and persistent than TBI in the mature brain. Understanding this developmental sensitivity is critical as children under four years of age sustain TBI more frequently than any other age group. Microglia (MG), resident immune cells of the brain that mediate neuroinflammation, are activated following TBI in the immature brain. However, the type and temporal profile of this activation and the consequences of altering it are still largely unknown. In a mouse model of closed head weight drop paediatric brain trauma, we characterized i) the temporal course of total cortical neuroinflammation and the phenotype of ex vivo isolated CD11B-positive microglia/macrophage (MG/MΦ) using a battery of 32 markers, and ii) neuropathological outcome 1 and 5days post-injury. We also assessed the effects of targeting MG/MΦ activation directly, using minocycline a prototypical microglial activation antagonist, on these processes and outcome. TBI induced a moderate increase in both pro- and anti-inflammatory cytokines/chemokines in the ipsilateral hemisphere. Isolated cortical MG/MΦ expressed increased levels of markers of endogenous reparatory/regenerative and immunomodulatory phenotypes compared with shams. Blocking MG/MΦ activation with minocycline at the time of injury and 1 and 2days post-injury had only transient protective effects, reducing ventricular dilatation and cell death 1day post-injury but having no effect on injury severity at 5days. This study demonstrates that, unlike in adults, the role of MG/MΦ in injury mechanisms following TBI in the immature brain may not be negative. An improved understanding of MG/MΦ function in paediatric TBI could support translational efforts to design therapeutic interventions.
Subject Neurology and Neuromuscular Diseases
Paediatrics
Keyword(s) Apoptosis
Cerebral
Chemokine
Cytokine
Immature
Inflammation
Macrophage
Minocycline
Neuron
Phenotype
DOI - identifier 10.1016/j.bbi.2016.11.001
Copyright notice © 2016 The Author(s). Published by Elsevier Inc.
ISSN 0889-1591
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 5 Abstract Views  -  Detailed Statistics
Created: Thu, 31 Jan 2019, 11:26:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us