Chorioamnionitis, neuroinflammation, and injury: Timing is key in the preterm ovine fetus

Gussenhoven, R, Westerlaken, R, Ophelders, D, Jobe, A, Kemp, M, Kallapur, S, Zimmermann, L, Sangild, P, Pankratova, S, Gressens, P, Kramer, B, Fleiss, B and Wolfs, T 2018, 'Chorioamnionitis, neuroinflammation, and injury: Timing is key in the preterm ovine fetus', Journal of Neuroinflammation, vol. 15, no. 1, pp. 1-13.

Document type: Journal Article
Collection: Journal Articles

Title Chorioamnionitis, neuroinflammation, and injury: Timing is key in the preterm ovine fetus
Author(s) Gussenhoven, R
Westerlaken, R
Ophelders, D
Jobe, A
Kemp, M
Kallapur, S
Zimmermann, L
Sangild, P
Pankratova, S
Gressens, P
Kramer, B
Fleiss, B
Wolfs, T
Year 2018
Journal name Journal of Neuroinflammation
Volume number 15
Issue number 1
Start page 1
End page 13
Total pages 13
Publisher BioMed Central Ltd.
Abstract BACKGROUND: Antenatal infection (i.e., chorioamnionitis) is an important risk factor for adverse neurodevelopmental outcomes after preterm birth. Destructive and developmental disturbances of the white matter are hallmarks of preterm brain injury. Understanding the temporal effects of antenatal infection in relation to the onset of neurological injury is crucial for the development of neurotherapeutics for preterm infants. However, these dynamics remain unstudied. METHODS: Time-mated ewes were intra-amniotically injected with lipopolysaccharide at 5, 12, or 24 h or 2, 4, 8, or 15 days before preterm delivery at 125 days gestational age (term ~ 150 days). Post mortem analyses for peripheral immune activation, neuroinflammation, and white matter/neuronal injury were performed. Moreover, considering the neuroprotective potential of erythropoietin (EPO) for perinatal brain injury, we evaluated (phosphorylated) EPO receptor (pEPOR) expression in the fetal brain following LPS exposure. RESULTS: Intra-amniotic exposure to this single bolus of LPS resulted in a biphasic systemic IL-6 and IL-8 response. In the developing brain, intra-amniotic LPS exposure induces a persistent microgliosis (IBA-1 immunoreactivity) but a shorter-lived increase in the pro-inflammatory marker COX-2. Cell death (caspase-3 immunoreactivity) was only observed when LPS exposure was greater than 8 days in the white matter, and there was a reduction in the number of (pre) oligodendrocytes (Olig2- and PDGFRα-positive cells) within the white matter at 15 days post LPS exposure only. pEPOR expression displayed a striking biphasic regulation following LPS exposure which may help explain contradicting results among clinical trials that tested EPO for the prevention of preterm brain injury. CONCLUSION: We provide increased understanding of the spatiotemporal pathophysiological changes in the preterm brain following intra-amniotic inflammation which may aid development of new interventions or implement in
Subject Neurology and Neuromuscular Diseases
Keyword(s) Brain injury
EPO receptor
DOI - identifier 10.1186/s12974-018-1149-x
Copyright notice © The Author(s). 2018
ISSN 1742-2094
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