Interaction of platelets with poly(vinylidene fluoride-co- hexafluoropropylene) electrospun surfaces

Ahmed, F, Choudhury, N, Dutta, N, Briteo E Abreu, S, Zannettino, A and Duncan, E 2014, 'Interaction of platelets with poly(vinylidene fluoride-co- hexafluoropropylene) electrospun surfaces', Biomacromolecules, vol. 15, no. 3, pp. 744-755.


Document type: Journal Article
Collection: Journal Articles

Title Interaction of platelets with poly(vinylidene fluoride-co- hexafluoropropylene) electrospun surfaces
Author(s) Ahmed, F
Choudhury, N
Dutta, N
Briteo E Abreu, S
Zannettino, A
Duncan, E
Year 2014
Journal name Biomacromolecules
Volume number 15
Issue number 3
Start page 744
End page 755
Total pages 12
Publisher American Chemical Society
Abstract Platelets are the major contributors in the process of thrombosis and in the failure of biomedical implants. A number of factors influence the platelet interaction with foreign surfaces such as surface morphology, surface chemistry, and adsorbed proteins. This study examined the effect of surface topography and chemistry of pristine and fibrinogen-adsorbed solvent cast (SC) and electrospun (ES) samples of poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) on platelet adhesion, activation, and aggregation. Qualitative and quantitative studies of fibrinogen adsorption were performed using time-of-flight secondary ion mass spectrometry (ToF-SIMS), while SEM, aggregometry, and liquid scintillation analyses were performed to evaluate platelet adhesion, aggregation, and serotonin release. While little or no platelet adhesion was observed on pristine ES surfaces, considerable adhesion, and measurable aggregation and serotonin release were observed on pristine SC surfaces. Notably, increased adhesion of platelets was observed following fibrinogen adsorption on SC surface with considerable aggregation and serotonin release compared with ES samples, where limited aggregation and platelet adhesion was observed. A further comparison of platelet adhesion, aggregation, and serotonin release was performed with plasma-adsorbed SC and ES surfaces. SC surfaces showed enhanced platelet adhesion, aggregation, and serotonin release compared to ES surfaces. This study shows that the morphology of samples plays a critical role on the biocompatibility of samples by altering the adsorption and adhesion of biomolecules and cells. The low level of adhesion, low aggregation, and serotonin release of platelets, even in the presence of fibrinogen and plasma-derived proteins, suggested that ES samples have the least thrombogenicity.
Subject Biomaterials
Chemical Engineering not elsewhere classified
DOI - identifier 10.1021/bm4015396
Copyright notice © 2014 American Chemical Society
ISSN 1525-7797
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