Self-organization, interfacial interaction and photophysical properties of gold nanoparticle complexes derived from resilin-mimetic fluorescent protein rec1-resilin

Mayavan, S, Dutta, N, Choudhury, N, Kim, M, Elvin, C and Hill, A 2011, 'Self-organization, interfacial interaction and photophysical properties of gold nanoparticle complexes derived from resilin-mimetic fluorescent protein rec1-resilin', Biomaterials, vol. 32, no. 11, pp. 2786-2796.


Document type: Journal Article
Collection: Journal Articles

Title Self-organization, interfacial interaction and photophysical properties of gold nanoparticle complexes derived from resilin-mimetic fluorescent protein rec1-resilin
Author(s) Mayavan, S
Dutta, N
Choudhury, N
Kim, M
Elvin, C
Hill, A
Year 2011
Journal name Biomaterials
Volume number 32
Issue number 11
Start page 2786
End page 2796
Total pages 11
Publisher Elsevier
Abstract In this investigation we report the synthesis of optically coupled hybrid architectures based on a new biomimetic fluorescent protein rec1- resilin and nanometer-scale gold nanoparticles (AuNPs) in a one-step method using a non-covalent mode of binding protocol. The presence of uniformly distributed fluorophore sequences, -Ser(Thr)-Tyr-Gly- along the molecular structure of rec1- resilin provides signnificant opportunity to synthesize fluorophore-modified AuNPs bioconjugates with unique photophysical properties. The detailed analyses of the AuNP-bioconjugates, synthesized under different experimental conditions using spectroscopic, microscopic and scattering techniques demonstrate the organizational pathways and the electronic and photophysical properties of the developed AuNP- rec1- resilin bioconjugates. The calculation of the bimolecular quenching constant using the Stern-Volmer equation confirms that the dominant mechanism involved in quenching of fluorescence of rec1- resilin in the presence of AuNP is static. Photoacoustic infrared spectroscopy was employed to understand the nature of the interfacial interaction between the AuNP and rec1- resilin and its evolution with pH. In such bioconjugates the quenched emission of fluorescence by AuNP on the fluorophore moiety of rec1- resilin in the immediate vicinity of the AuNP has significant potential for fluorescence-based detection schemes, sensors and also can be incorporated into nanoparticle-based devices.
Subject Chemical Engineering not elsewhere classified
Interdisciplinary Engineering not elsewhere classified
Nanobiotechnology
Keyword(s) Biomimetic protein
Gold nanoparticles
Gold-protein bioconjugates
Photophysical properties of bioconjugates
Self-assembly
DOI - identifier 10.1016/j.biomaterials.2010.12.030
Copyright notice © 2011 Elsevier Ltd. All rights reserved.
ISSN 0142-9612
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 17 Abstract Views  -  Detailed Statistics
Created: Thu, 31 Jan 2019, 11:26:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us