Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement

Thu, V, Tien, B, Nga, D, Thanh, L, Sinh, L, Le, T and Lam, T 2018, 'Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement', RSC Advances, vol. 8, no. 45, pp. 25361-25367.


Document type: Journal Article
Collection: Journal Articles

Title Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement
Author(s) Thu, V
Tien, B
Nga, D
Thanh, L
Sinh, L
Le, T
Lam, T
Year 2018
Journal name RSC Advances
Volume number 8
Issue number 45
Start page 25361
End page 25367
Total pages 7
Publisher Royal Society of Chemistry
Abstract The development of low cost, portable diagnostic tools for in-field detection of viruses and other pathogenic microorganisms is in great demand but remains challenging. In this study, a novel approach based on reduced graphene oxide-polyaniline (rGO-PANi) film for the in situ detection of loop-mediated-isothermal-amplification (LAMP) products by means of open circuit potential measurement is proposed. The pH-sensitive conducting polymer PANi was electro-deposited onto rGO coated screen printed electrodes and tuned to be at the emeraldine state at which the pH sensitivity was maximized. By combining PANi and rGO, the pH sensitivity of the system was modulated up to about -64 mV per pH unit. This enabled the number of amplified amplicons resulting from the isothermal amplification process to be monitored. The sensor was then examined for monitoring LAMP reactions using Hepatitis B virus (HBV) as a model. This simple, low-cost, reproducible and sensitive interfacing layer is expected to provide a new possibility for designing point-of-care sensors under limited-resource conditions.
Subject Physical Chemistry of Materials
Functional Materials
DOI - identifier 10.1039/c8ra04050h
Copyright notice This journal is © The Royal Society of Chemistry 2018
ISSN 2046-2069
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