Fast and Highly Sensitive Detection of Pathogens Wreathed with Magnetic Nanoparticles Using Dark-Field Microscopy

Chen, F, Tang, F, Yang, C, Zhao, X, Wang, J, Thierry, B, Bansal, V, Dai, J and Zhou, X 2018, 'Fast and Highly Sensitive Detection of Pathogens Wreathed with Magnetic Nanoparticles Using Dark-Field Microscopy', ACS Sensors, vol. 3, no. 10, pp. 2175-2181.


Document type: Journal Article
Collection: Journal Articles

Title Fast and Highly Sensitive Detection of Pathogens Wreathed with Magnetic Nanoparticles Using Dark-Field Microscopy
Author(s) Chen, F
Tang, F
Yang, C
Zhao, X
Wang, J
Thierry, B
Bansal, V
Dai, J
Zhou, X
Year 2018
Journal name ACS Sensors
Volume number 3
Issue number 10
Start page 2175
End page 2181
Total pages 7
Publisher American Chemical Society
Abstract Cryptosporidium parvum (C. parvum) is a highly potent zoonotic pathogen, which can do significant harm to both human beings and livestock. However, existing technologies or methods are deficient for rapid on-site detection of water contaminated with C. parvum. Better detection approaches are needed to allow water management agencies to stop major breakouts of the pathogen. Herein, we present a novel detection method for cryptosporidium in a tiny drop of sample using a magnetic nanoparticle (MNP) probe combined with dark-field microscopy in 30 min. The designed MNP probes bind with high affinity to C. parvum, resulting in the formation of a golden garland-like structure under dark-field microscopy. This MNP-based dark-field counting strategy yields an amazing PCR-like sensitivity of 8 attomolar (aM) (5 pathogens in 1 mu L). Importantly, the assay is very rapid (similar to 30 min) and is very simple to perform as it involves only one step of mixing and magnetic separation, followed by dropping on a slide for counting under dark-field microscope. By combining the advantages of the specific light-scattering characteristic of MNP probe under dark field and the selective magnetic separation ability of functionalized MNP, the proposed MNP-based dark-field enumeration method offers low cost and significant translational potential.
Subject Sensor Technology (Chemical aspects)
Nanobiotechnology
Keyword(s) Cryptosporidium parvum
dark-field microscope
garland-like structure
magnetic nanoparticles
pathogens detection
DOI - identifier 10.1021/acssensors.8b00785
Copyright notice © 2018 American Chemical Society
ISSN 2379-3694
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