The transcriptomic response of Streptococcus pneumoniae following exposure to cigarette smoke extract

Manna, S, Waring, A, Papanicolaou, A, Hall, N, Bozinovski, S, Dunne, E and Satzke, C 2018, 'The transcriptomic response of Streptococcus pneumoniae following exposure to cigarette smoke extract', Scientific Reports, vol. 8, pp. 1-12.


Document type: Journal Article
Collection: Journal Articles

Title The transcriptomic response of Streptococcus pneumoniae following exposure to cigarette smoke extract
Author(s) Manna, S
Waring, A
Papanicolaou, A
Hall, N
Bozinovski, S
Dunne, E
Satzke, C
Year 2018
Journal name Scientific Reports
Volume number 8
Start page 1
End page 12
Total pages 12
Publisher Nature
Abstract Exposure to cigarette smoke is a risk factor for respiratory diseases. Although most research has focused on its effects on the host, cigarette smoke can also directly affect respiratory pathogens, in some cases enhancing virulence. Streptococcus pneumoniae (the pneumococcus) is a leading cause of community-acquired pneumonia worldwide, however data on the effects of cigarette smoke on the pneumococcus are sparse. Using RNA-seq, we show that pneumococci exposed to cigarette smoke extract in a concentrated acute exposure in vitro model initiate a 'survival' transcriptional response including the upregulation of detoxification enzymes, efflux pumps and osmoregulator transporters, as well as the downregulation of fatty acid and D-alanyl lipoteichoic acid biosynthesis genes. Except for the downregulation of the pneumolysin gene, there were no changes in the expression of major virulence factors following exposure to cigarette smoke. Compared to unexposed pneumococci, smoke-exposed pneumococci did not exhibit any changes in viability, adherence, hydrophobicity or cell lysis susceptibility. In this study, we demonstrate that pneumococci adapt to acute noxious cigarette smoke exposure by inducing a gene expression signature that allows the bacteria to resist its harmful effects.
Subject Respiratory Diseases
Keyword(s) invasive pneumococcal disease
biofilm formation
epithelial-cells
staphylococcus-aureus
competence
virulence
stress
bacteria
choline
colonization
DOI - identifier 10.1038/s41598-018-34103-5
Copyright notice © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/
ISSN 2045-2322
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