Identifying Trends in Gold Nanoparticle Toxicity and Uptake: Size, Shape, Capping Ligand, and Biological Corona

Carnovale, C, Bryant, G, Shukla, R and Bansal, V 2019, 'Identifying Trends in Gold Nanoparticle Toxicity and Uptake: Size, Shape, Capping Ligand, and Biological Corona', ACS Omega, vol. 4, no. 1, pp. 242-256.


Document type: Journal Article
Collection: Journal Articles

Title Identifying Trends in Gold Nanoparticle Toxicity and Uptake: Size, Shape, Capping Ligand, and Biological Corona
Author(s) Carnovale, C
Bryant, G
Shukla, R
Bansal, V
Year 2019
Journal name ACS Omega
Volume number 4
Issue number 1
Start page 242
End page 256
Total pages 15
Publisher American Chemical Society
Abstract The drive behind the growing interest in understanding gold nanoparticle (AuNP) cytotoxicity originates from the promise of AuNPs for diverse biological applications across the fields of drug delivery, biosensing, biological imaging, gene therapy, and photothermal therapy. Although we continue to investigate the novel biomedical applications of AuNPs, progress is currently stalled at the periphery of understanding the forces that govern critical nano-bio interactions. In this work, we systematically probe the size, shape, and surface capping effects of nanogold by designing a set of eight unique AuNPs. This allowed us to undertake a systematic study involving each of these parameters in the context of their influence on the cytotoxicity and cellular uptake by human prostate cancer cells (PC3) as a model biological system. While studying the influence of these parameters, our study also investigated the influence of serum proteins in forming different levels of biological corona on AuNPs, thereby further influencing the nano-bio interface. As such, increased cellular uptake (by nanoparticle number) was observed with decreasing the AuNP size and increased uptake levels were observed for gold nanospheres (of the same size) stabilized with amino acids compared to citrate or cetyltrimethylammonium bromide (CTAB). Spherical particles were found to be taken up in greater numbers compared to the shapes with broad flat faces. When measuring cytotoxicity, CTAB-stabilized rod- and cube-shaped particles were well tolerated by the cells, whereas toxicity was observed in the case of CTAB-stabilized spherical and prismatic particles. These effects, however, are underpinned by different mechanisms. Further, it is demonstrated that it is possible for different chemical stabilizers to elicit varied cytotoxic effects. Although we find the limited role of serum proteins in mediating toxicity, they do play a critical role in influencing the cellular upt
Subject Nanobiotechnology
DOI - identifier 10.1021/acsomega.8b03227
Copyright notice © 2019 American Chemical Society
ISSN 2470-1343
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