Modulation of colonic hydrogen sulfide production by diet and mesalazine utilizing a novel gas-profiling technology

Yao, C, Rotbart, A, Ou, J, Kalantar Zadeh, K, Muir, J and Gibson, P 2018, 'Modulation of colonic hydrogen sulfide production by diet and mesalazine utilizing a novel gas-profiling technology', Gut Microbes, vol. 9, no. 6, pp. 510-522.


Document type: Journal Article
Collection: Journal Articles

Title Modulation of colonic hydrogen sulfide production by diet and mesalazine utilizing a novel gas-profiling technology
Author(s) Yao, C
Rotbart, A
Ou, J
Kalantar Zadeh, K
Muir, J
Gibson, P
Year 2018
Journal name Gut Microbes
Volume number 9
Issue number 6
Start page 510
End page 522
Total pages 13
Publisher Taylor & Francis Inc.
Abstract Excessive hydrogen sulfide (H2S) production from gut microbial metabolism may have clinically important relevance in the pathogenesis of gut disorders, including ulcerative colitis. However, little is known regarding factors that alter its production. Using a newly-designed in vitro gas-profiling technology, the study aimed to verify real-time H2S measurement reproducibility and thereafter, assess its production following exposure to dietary factors and 5-aminosalicylate acid (5-ASA). Measurements of H2S, carbon dioxide, hydrogen and methane measurements were compared between gas-profiling systems. Homogenized slurries were prepared from freshly-passed healthy human feces. Fifty ml slurries were aliquoted into separate fermentation chambers and substrates added including 1 g highly fermentable fructo-oligosaccharides (FOS) or resistant starch Hi-Maize (RS), or minimally fermentable psyllium or sterculia, 1 g cysteine, 0.9 g sodium sulfate or 1.2 mL of 1 M 5-ASA alone or in combinations. H2S release was sampled every 5 mins over 4-h and expressed relative to unspiked controls. RS suppressed H2S production by a mean 89.0 (SEM 4.8)% and FOS by 82.2 (6.2)% compared to <35 (17)% by psyllium and sterculia (p<0.001, two-way ANOVA). Cysteine stimulated H2S production by 1557 (532)%. The addition of FOS to slurries containing cysteine significantly suppressed H2S by 90 (2)% over the addition of 5-ASA (0.3 (2)%, p<0.001). Sulfate and 5-ASA had minimal overall effects. In conclusion, the H2S-profiling technology is a reproducible tool. Production of H2S is greatly enhanced by sulfur-amino acids but not inorganic sulfate, and is effectively suppressed by readily fermentable fibers. These findings inform potential designs of dietary therapies to reduce H2S production in vivo.
Subject Biomedical Instrumentation
Keyword(s) fiber
Hydrogen sulfide
microbiota
protein
sulfur
ulcerative colitis
DOI - identifier 10.1080/19490976.2018.1451280
Copyright notice © 2018 Taylor & Francis
ISSN 1949-0976
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