Characterization of diffuse intrinsic pontine glioma radiosensitivity using synchrotron microbeam radiotherapy and conventional radiation therapy in vitro

Smyth, L, Rogers, P, Crosbie, J and Donoghue, J 2018, 'Characterization of diffuse intrinsic pontine glioma radiosensitivity using synchrotron microbeam radiotherapy and conventional radiation therapy in vitro', Radiation Research, vol. 189, no. 2, pp. 146-155.


Document type: Journal Article
Collection: Journal Articles

Title Characterization of diffuse intrinsic pontine glioma radiosensitivity using synchrotron microbeam radiotherapy and conventional radiation therapy in vitro
Author(s) Smyth, L
Rogers, P
Crosbie, J
Donoghue, J
Year 2018
Journal name Radiation Research
Volume number 189
Issue number 2
Start page 146
End page 155
Total pages 10
Publisher Radiation Research Society
Abstract Synchrotron microbeam radiation therapy is a promising preclinical radiotherapy modality that has been proposed as an alternative to conventional radiation therapy for diseases such as diffuse intrinsic pontine glioma (DIPG), a devastating pediatric tumor of the brainstem. The primary goal of this study was to characterize and compare the radiosensitivity of two DIPG cell lines (SF7761 and JHH-DIPG-1) to microbeam and conventional radiation. We hypothesized that these DIPG cell lines would exhibit differential responses to each radiation modality. Single cell suspensions were exposed to microbeam (112, 250, 560, 1,180 Gy peak dose) or conventional (2, 4, 6 and 8 Gy) radiation to produce clonogenic cell-survival curves. Apoptosis induction and the cell cycle were also analyzed five days postirradiation using flow cytometry. JHH-DIPG-1 cells displayed greater radioresistance than SF7761 to both microbeam and conventional radiation, with higher colony formation and increased accumulation of G2/M-phase cells. Apoptosis was significantly increased in SF7761 cells compared to JHH-DIPG-1 after microbeam irradiation, demonstrating cell-line specific differential radiosensitivity to microbeam radiation. Additionally, biologically equivalent doses to microbeam and conventional radiation were calculated based on clonogenic survival, furthering our understanding of the response of cancer cells to these two radiotherapy modalities.
Subject Physical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Medical and Health Sciences not elsewhere classified
DOI - identifier 10.1667/RR4633.1
Copyright notice © 2018 by Radiation Research Society. All rights of reproduction in any form reserved.
ISSN 0033-7587
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 17 Abstract Views  -  Detailed Statistics
Created: Thu, 21 Feb 2019, 12:10:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us