A simple microfluidic chip design for fundamental bioseparation

Chan, A, Danquah, M, Agyei, D, Hartley, P and Zhu, Y 2014, 'A simple microfluidic chip design for fundamental bioseparation', Journal of Analytical Methods in Chemistry, vol. 2014, pp. 1-6.


Document type: Journal Article
Collection: Journal Articles

Title A simple microfluidic chip design for fundamental bioseparation
Author(s) Chan, A
Danquah, M
Agyei, D
Hartley, P
Zhu, Y
Year 2014
Journal name Journal of Analytical Methods in Chemistry
Volume number 2014
Start page 1
End page 6
Total pages 6
Publisher Hindawi
Abstract A microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to verify the performance of the chip. A mixture of dyes (fluorescein and rhodamine B) and a biopolymer mixture (10 kDa Dextran and 66 kDa BSA) were separated and the fluorescence technique was employed to detect the movement of the molecules. Fluorescein molecule was a nonretained species and rhodamine B was attached onto silica surface when dye mixture in deionized water was injected into the microchannel. The retention times for dextran molecule and BSA molecule in biopolymer separation experiment were 45 s and 120 s, respectively. Retention factor was estimated to be 3.3 for dextran and 10.4 for BSA. The selectivity was 3.2 and resolution was 10.7. Good separation of dyes and biopolymers was achieved and the chip design was verified.
Subject Chemical Engineering not elsewhere classified
Keyword(s) Performance Liquid-Chromatography
Solid-Phase Extraction
Methacrylate Monoliths
Surface Modification
Capillary Columns
Silica Particles
Stationary-Phase
Peptides
Proteins
Beads
DOI - identifier 10.1155/2014/175457
Copyright notice Copyright © 2014 Alan S. Chan et al.
ISSN 2090-8865
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