Marine microbial gene abundance and community composition in response to ocean acidification and elevated temperature in two contrasting coastal marine sediments

Currie, A, Tait, K, Parry, H, de Francisco-Mora, B, Hicks, N, Osborn, A, Widdicombe, S and Stahl, H 2017, 'Marine microbial gene abundance and community composition in response to ocean acidification and elevated temperature in two contrasting coastal marine sediments', Frontiers in Microbiology, vol. 8, pp. 1-10.


Document type: Journal Article
Collection: Journal Articles

Title Marine microbial gene abundance and community composition in response to ocean acidification and elevated temperature in two contrasting coastal marine sediments
Author(s) Currie, A
Tait, K
Parry, H
de Francisco-Mora, B
Hicks, N
Osborn, A
Widdicombe, S
Stahl, H
Year 2017
Journal name Frontiers in Microbiology
Volume number 8
Start page 1
End page 10
Total pages 10
Publisher Frontiers Research Foundation
Abstract Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidification for benthic microbial communities, which drive sedimentary biogeochemical cycles. In this research, mesocosm experiments were set up using muddy and sandy coastal sediments to investigate the independent and interactive effects of elevated carbon dioxide concentrations (750 ppm CO 2 ) and elevated temperature (ambient +4 ◦ C) on the abundance of taxonomic and functional microbial genes. Specific quantitative PCR primers were used to target archaeal, bacterial, and cyanobacterial/chloroplast 16S rRNA in both sediment types. Nitrogen cycling genes archaeal and bacterial ammonia monooxygenase (amoA) and bacterial nitrite reductase (nirS) were specifically targeted to identify changes in microbial gene abundance and potential impacts on nitrogen cycling. In muddy sediment, microbial gene abundance, including amoA and nirS genes, increased under elevated temperature and reduced under elevated CO 2 after 28 days, accompanied by shifts in community composition. In contrast, the combined stressor treatment showed a non-additive effect with lower microbial gene abundance throughout the experiment. The response of microbial communities in the sandy sediment was less pronounced, with the most noticeable response seen in the archaeal gene abundances in response to environmental stressors over time. 16S rRNA genes (amoA and nirS) were lower in abundance in the combined stressor treatments in sandy sediments. Our results indicated that marine benthic microorganisms, especially in muddy sediments, are susceptible to changes in ocean carbonate chemistry and seawater temperature, which ultimately may have an impact upon key benthic biogeochemical cycles.
Subject Environmental Science and Management not elsewhere classified
Keyword(s) Ammonia-oxidizing bacteria
Denitrifying bacteria
Microbial community
Muddy sediment
Ocean acidification
Ocean warming
Sandy sediment
DOI - identifier 10.3389/fmicb.2017.01599
Copyright notice © 2017 The Author(s). Creative Commons Attribution License.
ISSN 1664-302X
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