Isolating plasma from blood using a dielectrophoresis-active hydrophoretic device

Yan, S, Zhang, J, Alici, G, Du, H, Zhu, Y and Li, W 2014, 'Isolating plasma from blood using a dielectrophoresis-active hydrophoretic device', Lab on a Chip, vol. 14, no. 16, pp. 2993-3003.


Document type: Journal Article
Collection: Journal Articles

Title Isolating plasma from blood using a dielectrophoresis-active hydrophoretic device
Author(s) Yan, S
Zhang, J
Alici, G
Du, H
Zhu, Y
Li, W
Year 2014
Journal name Lab on a Chip
Volume number 14
Issue number 16
Start page 2993
End page 3003
Total pages 11
Publisher Royal Society of Chemistry
Abstract Plasma is a complex substance that contains proteins and circulating nucleic acids and viruses that can be utilised for clinical diagnostics, albeit a precise analysis depends on the plasma being totally free of cells. We proposed the use of a dielectrophoresis (DEP)-active hydrophoretic method to isolate plasma from blood in a high-throughput manner. This microfluidic device consists of anisotropic microstructures embedded on the top of the channel which generate lateral pressure gradients while interdigitised electrodes lay on the bottom of the channel which can push particles or cells into a higher level using a negative DEP force. Large and small particles or cells (3 μm and 10 μm particles, and red blood cells, white blood cells, and platelets) can be focused at the same time in our DEP-active hydrophoretic device at an appropriate flow rate and applied voltage. Based on this principle, all the blood cells were filtrated from whole blood and then the plasma was extracted with a purity of 94.2% and a yield of 16.5% at a flow rate of 10 μL min -1 . This solved the challenging problem caused by the relatively low throughput of the DEP based device. Our DEP-active hydrophoretic device is a flexible and tunable system that can control the lateral positions of particles by modulating the external voltages without redesigning and fabricating a new channel, and because it is easy to operate, it is easily compatible with other microfluidic platforms that are used for further detection.
Subject Biomedical Engineering not elsewhere classified
Keyword(s) Microfluidic Device
Whole-Blood
Separation
Microchannel
Filtration
Particles
Microparticles
Obstacles
Channel
Arrays
DOI - identifier 10.1039/c4lc00343h
Copyright notice © 2014 The Royal Society of Chemistry
ISSN 1473-0197
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