A rapid assay for Hendra virus IgG antibody detection and its titre estimation using magnetic nanoparticles and phycoerythrin

Gao, Y, Pallister, J, Lapierre, F, Crameri, G, Wang, L and Zhu, Y 2015, 'A rapid assay for Hendra virus IgG antibody detection and its titre estimation using magnetic nanoparticles and phycoerythrin', Journal of Virological Methods, vol. 222, pp. 170-177.


Document type: Journal Article
Collection: Journal Articles

Title A rapid assay for Hendra virus IgG antibody detection and its titre estimation using magnetic nanoparticles and phycoerythrin
Author(s) Gao, Y
Pallister, J
Lapierre, F
Crameri, G
Wang, L
Zhu, Y
Year 2015
Journal name Journal of Virological Methods
Volume number 222
Start page 170
End page 177
Total pages 8
Publisher Elsevier
Abstract Detection of Hendra viral IgG antibody in animal sera is useful for surveillance following a virus outbreak. The commonly used enzyme-linked immunosorbent assay and fluorescence-based Luminex assay typically consist of three steps and take at least several hours to complete. We have simplified the procedure to two steps in an effort to develop a rapid procedure for IgG antibody, but not IgM antibody, detection. This is achieved by conjugating the fluorescence label R-phycoerythrin directly onto the IgG binding protein Protein G. The use of magnetic nanoparticles, due to their large specific surface area, has helped reduce each of the binding steps to 20. min. As a result, the whole assay can be completed in 60. min. We also demonstrate a method to quickly estimate IgG antibody titres by assaying the sera at only two dilutions (i.e. 1:20 and 1:1000) and using the fluorescence ratio at these dilutions as an indicator of antibody titre. The results of this approach correlated well with the well-regarded serum neutralization test in virus antibody assays. This protocol reported here can be adopted in Luminex assays, fluorescence-linked immunosorbent assays and assays on microfluidics platforms for rapid antibody surveillance of Hendra and other viruses.
Subject Medical Biotechnology not elsewhere classified
Keyword(s) Antibody
Hendra virus
Immunoassay
Magnetic nanoparticles
Phycoerythrin
DOI - identifier 10.1016/j.jviromet.2015.05.008
Copyright notice © 2015 Elsevier B.V.
ISSN 0166-0934
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