Dissecting the genetic basis for seed coat mucilage heteroxylan biosynthesis in plantago ovata using gamma irradiation and infrared spectroscopy

Tucker, M, Ma, C, Phan, J, Neumann, K, Shirley, N, Hahn, M, Cozzolino, D and Burton, R 2017, 'Dissecting the genetic basis for seed coat mucilage heteroxylan biosynthesis in plantago ovata using gamma irradiation and infrared spectroscopy', Frontiers in Plant Science, vol. 8, pp. 1-14.


Document type: Journal Article
Collection: Journal Articles

Title Dissecting the genetic basis for seed coat mucilage heteroxylan biosynthesis in plantago ovata using gamma irradiation and infrared spectroscopy
Author(s) Tucker, M
Ma, C
Phan, J
Neumann, K
Shirley, N
Hahn, M
Cozzolino, D
Burton, R
Year 2017
Journal name Frontiers in Plant Science
Volume number 8
Start page 1
End page 14
Total pages 14
Publisher Frontiers Research Foundation
Abstract Seeds from the myxospermous species Plantago ovata release a polysaccharide-rich mucilage upon contact with water. This seed coat derived mucilage is composed predominantly of heteroxylan (HX) and is utilized as a gluten-free dietary fiber supplement to promote human colorectal health. In this study, a gamma-irradiated P. ovata population was generated and screened using histological stains and Fourier Transform Mid Infrared (FTMIR) spectroscopy to identify putative mutants showing defects in seed coat mucilage HX composition and/or structure. FTMIR analysis of dry seed revealed variation in regions of the IR spectra previously linked to xylan structure in Secale cereale (rye). Subsequent absorbance ratio and PCA multivariate analysis identified 22 putative mutant families with differences in the HX IR fingerprint region. Many of these showed distinct changes in the amount and subtle changes in structure of HX after mucilage extrusion, while 20% of the putative HX mutants identified by FTMIR showed no difference in staining patterns of extruded mucilage compared to wildtype. Transcriptional screening analysis of two putative reduced xylan in mucilage (rxm) mutants, rxm1 and rxm3, revealed that changes in HX levels in rxm1 correlate with reduced transcription of known and novel genes associated with xylan synthesis, possibly indicative of specific co-regulatory units within the xylan biosynthetic pathway. These results confirm that FTMIR is a suitable method for identifying putative mutants with altered mucilage HX composition in P. ovata, and therefore forms a resource to identify novel genes involved in xylan biosynthesis.
Subject Quality Assurance, Chemometrics, Traceability and Metrological Chemistry
Keyword(s) Plantago
mucilage
xylan
spectroscopy
seed
mutagenesis
DOI - identifier 10.3389/fpls.2017.00326
Copyright notice © 2017 Tucker, Ma, Phan, Neumann, Shirley, Hahn, Cozzolino and Burton.
ISSN 1664-462X
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