Sex and vaccination: Insights from female rats vaccinated with juvenile-specific proteases from Fasciola hepatica

Wesołowska, A, Basałaj, K, Norbury, L, Sielicka, A, Wędrychowicz, H and Zawistowska-Deniziak, A 2018, 'Sex and vaccination: Insights from female rats vaccinated with juvenile-specific proteases from Fasciola hepatica', Veterinary Parasitology, vol. 255, pp. 91-96.

Document type: Journal Article
Collection: Journal Articles

Title Sex and vaccination: Insights from female rats vaccinated with juvenile-specific proteases from Fasciola hepatica
Author(s) Wesołowska, A
Basałaj, K
Norbury, L
Sielicka, A
Wędrychowicz, H
Zawistowska-Deniziak, A
Year 2018
Journal name Veterinary Parasitology
Volume number 255
Start page 91
End page 96
Total pages 6
Publisher Elsevier
Abstract Most animal research is less evidence-based for females, with the majority of studies conducted on males. Since immune responses vary between males and females, sexual dimorphism in immunity contributes, among other things, to sex-based differences post-vaccination. However, the issue of sex effects in animal vaccine research is rarely considered in vaccine study design. Previously, we have evaluated the efficacy of cathepsin L3 (FhCL3-1 and FhCL3-2) and B3 proteases (FhCB3) from juvenile Fasciola hepatica as vaccines against fasciolosis in male rats. Their administration resulted in reductions in liver fluke recovery in the range of 4763% when compared with an infection control group. Here, we investigated if the protective effect of vaccination with these proteins can also be observed for female rats. The data indicates females were not protected from F. hepatica infection when vaccinated with juvenile cathepsins. Only in the FhCL3-2 vaccinated group was a low, non-significant, reduction in worm burden observed (21%). Although liver fluke mean body lengths and wet weights were reduced in vaccinated animals when compared with the infection controls, these effects were adjuvant- not vaccine-induced, while for males changes in these parameters were related primarily to vaccination. Specific humoral responses throughout the study were evident; however, trends in antibody responses in females replicated trends observed previously for male humoral responses. Formerly, elevated levels of FhCL3-1 and FhCL3-2 specific IgG1 and IgG2a were suggested to be correlated with protection. Here, despite increased and clear responses of these antibodies, protection was not observed. Hence, in the present study the roles of IgG1 and IgG2 in liver fluke reduction are questionable. Results demonstrated in our study show that observations obtained in one sex are not always applicable to the other sex. Hopefully, the findings of the study will stimulate discussion of the issue of sex im
Subject Microbiology not elsewhere classified
Fisheries Sciences not elsewhere classified
Veterinary Sciences not elsewhere classified
Keyword(s) Cathepsin B3
Cathepsin L3
Fasciola hepatica
Juvenile-Specific antigens
Multivalent vaccine
DOI - identifier 10.1016/j.vetpar.2018.04.001
Copyright notice © 2018 Elsevier B.V. All rights reserved.
ISSN 0304-4017
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