Biological hydrogen peroxide detection with aryl boronate and benzil BODIPY-based fluorescent probes

Purdey, M, McLennan, H, Sutton-McDowall, M, Drumm, D, Zhang, X, Capon, P, Heng, S, Thompson, J and Abell, A 2018, 'Biological hydrogen peroxide detection with aryl boronate and benzil BODIPY-based fluorescent probes', Sensors and Actuators, B: Chemical, vol. 262, pp. 750-757.


Document type: Journal Article
Collection: Journal Articles

Title Biological hydrogen peroxide detection with aryl boronate and benzil BODIPY-based fluorescent probes
Author(s) Purdey, M
McLennan, H
Sutton-McDowall, M
Drumm, D
Zhang, X
Capon, P
Heng, S
Thompson, J
Abell, A
Year 2018
Journal name Sensors and Actuators, B: Chemical
Volume number 262
Start page 750
End page 757
Total pages 8
Publisher Elsevier
Abstract The detection of hydrogen peroxide (H2O2) using fluorescent probes is critical to the study of oxidative stress in biological environments. Two important sensing architectures for detecting H2O2, aryl boronates and benzils, are compared here using novel boron-dipyrromethene (BODIPY) fluorescent probes. The aryl boronate PeroxyBODIPY-1 (PB1) and benzil-based nitrobenzoylBODIPY (NbzB) were synthesised from a common BODIPY intermediate in order to compare sensitivity and selectivity to H2O2. The aryl boronate PB1 gives the highest change in fluorescence on reaction with H2O2 while the benzil NbzB exhibits exclusive selectivity for H2O2 over other reactive oxygen species (ROS). Both proved to be cell-permeable, with PB1 being able to detect H2O2 in denuded bovine oocytes. The strengths of these aryl boronate and benzil probes can now be exploited concurrently to elucidate biological mechanisms of H2O2 production and oxidative stress.
Subject Optical Physics not elsewhere classified
Materials Engineering not elsewhere classified
Analytical Chemistry not elsewhere classified
Keyword(s) BODIPY
Fluorescent sensor
Hydrogen peroxide
Reactive oxygen species
DOI - identifier 10.1016/j.snb.2018.01.198
Copyright notice © 2018 Elsevier B.V. All rights reserved.
ISSN 0925-4005
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