Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler's murine encephalomyelitis virus

Ma, L, Cong, F, Zhu, Y, Wu, M, Xu, F, Huang, R, Moore, R and Guo, P 2018, 'Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler's murine encephalomyelitis virus', Molecular and Cellular Probes, vol. 41, pp. 27-31.


Document type: Journal Article
Collection: Journal Articles

Title Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler's murine encephalomyelitis virus
Author(s) Ma, L
Cong, F
Zhu, Y
Wu, M
Xu, F
Huang, R
Moore, R
Guo, P
Year 2018
Journal name Molecular and Cellular Probes
Volume number 41
Start page 27
End page 31
Total pages 5
Publisher Academic Press
Abstract Theiler's murine encephalomyelitis virus (TMEV) is one of the most common viral pathogens that circulate widely in captive mouse colonies. A molecular biology detection method would be a useful tool to use in an integrated program to monitor and prevent TMEV infection and transmission. Thus, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed to detect TMEV infection. The sensitivity of the RT-RPA assay approached 8 copies per reaction, which is equivalent to the sensitivity of RT-qPCR reactions. This assay did not detect RNA extracts from other murine pathogens included in this study or TMEV negative samples. Brain tissues and contaminated biological materials were used to assess the clinical performance of the RT-RPA. The detection results of RT-RPA and RT-qPCR were very similar, except that a contaminated biological material sample which was positive by RT-qPCR, with a CT value of 38, was negative by RT-RPA. In summary, the developed RT-RPA assay offers a rapid, sensitive and specific alternative method for monitoring of TMEV, especially in resource-limited conditions.
Subject Infectious Agents
Medical Biotechnology Diagnostics (incl. Biosensors)
Keyword(s) Detection
Recombinase polymerase amplification
RT-qPCR
Theiler's murine encephalomyelitis virus
DOI - identifier 10.1016/j.mcp.2018.08.006
Copyright notice © 2018 Elsevier Ltd. All rights reserved.
ISSN 0890-8508
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