Evaluation of the use of attenuated total reflectance mid infrared spectroscopy to determine fatty acids in intact seeds of barley (Hordeum vulgare)

Cozzolino, D, Roumeliotis, S and Eglinton, J 2014, 'Evaluation of the use of attenuated total reflectance mid infrared spectroscopy to determine fatty acids in intact seeds of barley (Hordeum vulgare)', LWT - Food Science and Technology, vol. 56, no. 2, pp. 478-483.


Document type: Journal Article
Collection: Journal Articles

Title Evaluation of the use of attenuated total reflectance mid infrared spectroscopy to determine fatty acids in intact seeds of barley (Hordeum vulgare)
Author(s) Cozzolino, D
Roumeliotis, S
Eglinton, J
Year 2014
Journal name LWT - Food Science and Technology
Volume number 56
Issue number 2
Start page 478
End page 483
Total pages 6
Publisher Academic Press
Abstract Attenuated total reflectance (ATR) mid-infrared (MIR) spectroscopy was evaluated to measure fatty acids (FA) and total lipids in intact seed barley (Hordeum vulgare) samples. Results from this study demonstrated the capability of ATR-MIR spectroscopy coupled with partial least squares (PLS) regression to measure FA non-destructively in intact barley seeds. The coefficients of determination in cross validation (R2) and the standard error of cross validation (SECV) were 0.76 and 0.004g100g-1 for stearic, 0.75 and 0.037g100g-1 for oleic, 0.45 and 0.02g100g-1 for palmitic acids, and 0.89 and 0.13g100g-1 for total lipids, respectively. The practical implications of this study showed that ATR-MIR spectroscopy confer valuable benefits for the grain industry and in particular in breeding programs as a non-destructive high throughput method. © 2013 Elsevier Ltd.
Subject Food Sciences not elsewhere classified
Keyword(s) Attenuated total reflectance
Barley
Fatty acids
Lipids
Mid infrared
DOI - identifier 10.1016/j.lwt.2013.11.019
Copyright notice © 2013 Elsevier
ISSN 0023-6438
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