Raman optical activity of tetra-alanine in the poly(l-proline) II type peptide conformation

Furuta, M, Fujisawa, T, Urago, H, Eguchi, T, Shingae, T, Takahashi, S, Blanch, E and Unno, M 2017, 'Raman optical activity of tetra-alanine in the poly(l-proline) II type peptide conformation', Physical Chemistry Chemical Physics, vol. 19, no. 3, pp. 2078-2086.


Document type: Journal Article
Collection: Journal Articles

Title Raman optical activity of tetra-alanine in the poly(l-proline) II type peptide conformation
Author(s) Furuta, M
Fujisawa, T
Urago, H
Eguchi, T
Shingae, T
Takahashi, S
Blanch, E
Unno, M
Year 2017
Journal name Physical Chemistry Chemical Physics
Volume number 19
Issue number 3
Start page 2078
End page 2086
Total pages 9
Publisher Royal Society of Chemistry
Abstract The poly(l-proline) II (PPII) helix is considered to be a major conformation in disordered polypeptides and unfolded proteins in aqueous solution. The PPII conformation can be identified by using Raman optical activity (ROA), which measures the different intensities of right- and left-circularly polarized Raman scattered light from chiral molecules and provides information on stereochemistry associated with vibrational motions. In the present study, we used tetra-alanine (Ala 4 ) as a model system, since its central amide bond adopts the PPII conformation. The predominance of the PPII conformation was supported by 11 ns molecular dynamics (MD) simulations at 300 K. The MD snapshots were used for subsequent quantum mechanical/molecular mechanical (QM/MM) calculations to compute the Raman and ROA spectra. The present MD + QM/MM analysis leads to a good agreement between the observed and simulated spectra, allowing us to assign most of the spectral features including the ROA band near 1320 cm -1 , which has been used as a marker for the PPII conformation. This positive ROA band has three components. The lower frequency component near 1310 cm -1 arises from an internal peptide bond, whereas the higher frequency components around 1320-1335 cm -1 appear due to N- and C-terminal peptide groups. The MD + QM/MM calculations also reproduced the electronic circular dichroism spectra of Ala 4 . The present results provide a satisfactory framework for future investigations of unfolded/disordered proteins as well as peptides in solutions by chiral spectroscopic methods.
Subject Physical Chemistry not elsewhere classified
DOI - identifier 10.1039/c6cp07828a
Copyright notice © 2017 the Owner Societies.
ISSN 1463-9076
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 8 Abstract Views  -  Detailed Statistics
Created: Tue, 26 Mar 2019, 09:36:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us