Rapid and specific methods to differentiate foodborne pathogens, campylobacter jejuni, campylobacter coli, and the new species causing spotty liver disease in chickens, campylobacter hepaticus

Van, T, Anwar, A, Scott, P and Moore, R 2018, 'Rapid and specific methods to differentiate foodborne pathogens, campylobacter jejuni, campylobacter coli, and the new species causing spotty liver disease in chickens, campylobacter hepaticus', Foodborne Pathogens and Disease, vol. 15, no. 8, pp. 526-530.


Document type: Journal Article
Collection: Journal Articles

Title Rapid and specific methods to differentiate foodborne pathogens, campylobacter jejuni, campylobacter coli, and the new species causing spotty liver disease in chickens, campylobacter hepaticus
Author(s) Van, T
Anwar, A
Scott, P
Moore, R
Year 2018
Journal name Foodborne Pathogens and Disease
Volume number 15
Issue number 8
Start page 526
End page 530
Total pages 5
Publisher Mary Ann Liebert, Inc. Publishers
Abstract Campylobacter jejuni and Campylobacter coli play a major role in bacteria-related foodborne illness in humans. Recently, a newly identified species, Campylobacter hepaticus, was shown to be the causative agent of spotty liver disease in chickens. The pathogenic potential of C. hepaticus in humans is unknown. This new species contains genes usually used to detect C. jejuni and C. coli in DNA-based detection methods, such as the hippuricase (hipO) gene and the glyA (serine hydroxymethyltransferase) gene, with a high degree of similarity. Therefore, polymerase chain reaction (PCR) primers used to detect these species need to be evaluated carefully to prevent misidentification of these important Campylobacter species. A multiplex PCR was developed and optimized to simultaneously and specifically identify the presence of C. jejuni, C. coli, and C. hepaticus in chicken samples containing high-complexity microbiota. The assay represents a new diagnostic tool for investigating the epidemiology of Campylobacter colonization in poultry and environmental samples. It may also be applicable to the investigation of Campylobacter contamination in food and in outbreaks of campylobacteriosis.
Subject Bacteriology
Keyword(s) Campylobacter jejuni
Campylobacter coli
Campylobacter hepaticus
methods
PCR
foodborne pathogens
DOI - identifier 10.1089/fpd.2017.2367
Copyright notice © Mary Ann Liebert, Inc.
ISSN 1535-3141
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 28 Abstract Views  -  Detailed Statistics
Created: Tue, 26 Mar 2019, 09:36:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us