Immunogenicity of a tripartite cell penetrating peptide containing a MUC1 variable number of tandem repeat (VNTR) and a T helper epitope

Brooks, N, Hsu, J, Esparon, S, Pouniotis, D and Pietersz, G 2018, 'Immunogenicity of a tripartite cell penetrating peptide containing a MUC1 variable number of tandem repeat (VNTR) and a T helper epitope', Molecules, vol. 23, no. 9, pp. 1-21.


Document type: Journal Article
Collection: Journal Articles

Title Immunogenicity of a tripartite cell penetrating peptide containing a MUC1 variable number of tandem repeat (VNTR) and a T helper epitope
Author(s) Brooks, N
Hsu, J
Esparon, S
Pouniotis, D
Pietersz, G
Year 2018
Journal name Molecules
Volume number 23
Issue number 9
Start page 1
End page 21
Total pages 21
Publisher MDPIAG
Abstract Peptide-based vaccines for cancer have many advantages however, for optimization these immunogens should incorporate peptide epitopes that induce CD8, as well as CD4 responses, antibody and long term immunity. Cell penetrating peptides (CPP) with a capacity of cytosolic delivery have been used to deliver antigenic peptides and proteins to antigen presenting cells to induce cytotoxic T cell, helper T cell and humoral responses in mice. For this study, a tripartite CPP including a mucin 1 (MUC1) variable number of tandem repeat (VNTR) containing multiple T cell epitopes and tetanus toxoid universal T helper epitope peptide (tetCD4) was synthesised (AntpMAPMUC1tet) and immune responses investigated in mice. Mice vaccinated with AntpMAPMUC1tet + CpG show enhanced antigen-specific interferon-gamma (IFN-γ) and IL-4 T cell responses compared with AntpMAPMUC1tet vaccination alone and induced a Th1 response, characterised by a higher ratio of IgG2a antibody/IgG1 antibodies. Furthermore, vaccination generated long term MUC1-specific antibody and T cell responses and delayed growth of MUC1+ve tumours in mice. This data demonstrates the efficient delivery of branched multiple antigen peptides incorporating CPP and that the addition of CpG augments immune responses.
Subject Medicinal and Biomolecular Chemistry not elsewhere classified
Keyword(s) Antigen delivery
Immunogenicity
Immunotherapy
Membrane penetrating peptide
Membrane translocating peptide
Mucin 1
Multiple antigen peptide
Penetratin
TLR agonist
Vaccine
DOI - identifier 10.3390/molecules23092233
Copyright notice © 2018 by the authors. Creative Commons Attribution (CC BY) license
ISSN 1420-3049
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