Overexpressing ovotransferrin and avian β-defensin-3 improves antimicrobial capacity of chickens and poultry products

Cooper, C, Tizard, M, Stanborough, T, Moore, S and Moore, R., et al., 2019, 'Overexpressing ovotransferrin and avian β-defensin-3 improves antimicrobial capacity of chickens and poultry products', Transgenic Research, vol. 28, no. 1, pp. 51-76.


Document type: Journal Article
Collection: Journal Articles

Title Overexpressing ovotransferrin and avian β-defensin-3 improves antimicrobial capacity of chickens and poultry products
Author(s) Cooper, C
Tizard, M
Stanborough, T
Moore, S
Moore, R., et al.,
Year 2019
Journal name Transgenic Research
Volume number 28
Issue number 1
Start page 51
End page 76
Total pages 26
Publisher Springer Netherlands
Abstract Zoonotic and foodborne diseases pose a significant burden, decreasing both human and animal health. Modifying chickens to overexpress antimicrobials has the potential to decrease bacterial growth on poultry products and boost chicken innate immunity. Chickens overexpressing either ovotransferrin or avian -defensin-3 (AvD3) were generated using Tol-2 transposons. Transgene expression at the RNA and protein level was seen in egg white, breast muscle, and serum. There were significant differences in the immune cell populations in the blood, bursa, and spleen associated with transgene expression including an increased proportion of CD8+ cells in the blood of ovotransferrin and AvD3 transgenic birds. Expression of the antimicrobials inhibited the in vitro growth of human and chicken bacterial pathogens and spoilage bacteria. For example, transgene expression significantly reduced growth of aerobic and coliform bacteria in breast muscle and decreased the growth of Salmonella enterica in egg white. Overall these results indicate that overexpression of antimicrobials in the chicken can impact the immune system and increase the antimicrobial capacity of poultry products.
Subject Animal Protection (Pests and Pathogens)
Keyword(s) Antimicrobials
Bacteria
Overexpression
Pathogens
Poultry
Transgenic
DOI - identifier 10.1007/s11248-018-0101-2
Copyright notice © 2018, Springer Nature Switzerland AG.
ISSN 0962-8819
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 16 Abstract Views  -  Detailed Statistics
Created: Tue, 26 Mar 2019, 09:36:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us