Double-locus sequence typing using porA and peb1A for epidemiological studies of Campylobacter jejuni

Ahmed, M, Dunn, L, Valcanis, M, Hogg, G and Ivanova, E 2014, 'Double-locus sequence typing using porA and peb1A for epidemiological studies of Campylobacter jejuni', Foodborne Pathogens and Disease, vol. 11, no. 3, pp. 194-199.


Document type: Journal Article
Collection: Journal Articles

Title Double-locus sequence typing using porA and peb1A for epidemiological studies of Campylobacter jejuni
Author(s) Ahmed, M
Dunn, L
Valcanis, M
Hogg, G
Ivanova, E
Year 2014
Journal name Foodborne Pathogens and Disease
Volume number 11
Issue number 3
Start page 194
End page 199
Total pages 6
Publisher Mary Ann Liebert Inc.
Abstract Campylobacter jejuni is the leading cause of foodborne bacterial gastroenteritis worldwide. Bacterial typing schemes play an important role in epidemiological investigations to trace the source and route of transmission of the infectious agent by identifying outbreak and differentiating among sporadic infections. In this study, a double-locus sequence typing (DLST) scheme for C. jejuni based on concatenated partial sequences of porA and peb1A genes is proposed. The DLST scheme was validated using 50 clinical and environmental C. jejuni strains isolated from human (C5, H, H15-H19), chicken (CH1-CH15), water (W2-W17), and ovine samples (OV1-OV6). The scheme was found to be highly discriminatory (discrimination index [DI]=0.964) and epidemiologically concordant based on C. jejuni strains studied. The DLST showed discriminatory power above 0.95 and excellent congruence to multilocus sequence typing and can be recommended as a rapid and low-cost typing scheme for epidemiological investigation of C. jejuni. It is suggested that the DLST scheme is suitable for identification of outbreak strains and differentiation of the sporadic infection strains.
Subject Bacteriology
DOI - identifier 10.1089/fpd.2013.1634
Copyright notice © Mary Ann Liebert, Inc.
ISSN 1535-3141
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