N,N'-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier

Ho, J, Al-Deen, F, AI-Abboodi, A, Selomulya, C, Xiang, S, Plebanski, M and Forde, G 2011, 'N,N'-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier', Colloids and Surfaces B: Biointerfaces, vol. 83, no. 1, pp. 83-90.

Document type: Journal Article
Collection: Journal Articles

Title N,N'-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier
Author(s) Ho, J
Al-Deen, F
AI-Abboodi, A
Selomulya, C
Xiang, S
Plebanski, M
Forde, G
Year 2011
Journal name Colloids and Surfaces B: Biointerfaces
Volume number 83
Issue number 1
Start page 83
End page 90
Total pages 8
Publisher Elsevier
Abstract Particulates with specific sizes and characteristics can induce potent immune responses by promoting antigen uptake of appropriate immuno-stimulatory cell types. Magnetite (Fe3O4) nanoparticles have shown many potential bioapplications due to their biocompatibility and special characteristics. Here, superparamagnetic Fe3O4 nanoparticles (SPIONs) with high magnetization value (70 emu g−1) were stabilized with trisodium citrate and successfully conjugated with a model antigen (ovalbumin, OVA) via N,N′-carbonyldiimidazole (CDI) mediated reaction, to achieve a maximum conjugation capacity at approximately 13 μg μm−2. It was shown that different mechanisms governed the interactions between the OVA molecules and magnetite nanoparticles at different pH conditions. We evaluated as-synthesized SPION against commercially available magnetite nanoparticles. The cytotoxicity of these nanoparticles was investigated using mammalian cells. The reported CDI-mediated reaction can be considered as a potential approach in conjugating biomolecules onto magnetite or other biodegradable nanoparticles for vaccine delivery.
Subject Immunology not elsewhere classified
Keyword(s) Magnetite
Particulate vaccine carrier
Superparamagnetic nanoparticles
DOI - identifier 10.1016/j.colsurfb.2010.11.001
Copyright notice © 2010 Elsevier B.V. All rights reserved.
ISSN 0927-7765
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