Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies

Ali, M, Kayani, A, Yeo, L, Chrimes, A, Ahmad, M, Ostrikov, K and Majlis, B 2018, 'Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies', Biomedical Microdevices, vol. 20, no. 4, pp. 1-13.


Document type: Journal Article
Collection: Journal Articles

Title Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies
Author(s) Ali, M
Kayani, A
Yeo, L
Chrimes, A
Ahmad, M
Ostrikov, K
Majlis, B
Year 2018
Journal name Biomedical Microdevices
Volume number 20
Issue number 4
Start page 1
End page 13
Total pages 13
Publisher Springer New York LLC
Abstract Cell contact formation, which is the process by which cells are brought into close proximity is an important biotechnological process in cell and molecular biology. Such manipulation is achieved by various means, among which dielectrophoresis (DEP) is widely used due to its simplicity. Here, we show the advantages in the judicious choice of the DEP microelectrode configuration in terms of limiting undesirable effects of dielectric heating on the cells, which could lead to their inactivation or death, as well as the possibility for cell clustering, which is particularly advantageous over the linear cell chain arrangement typically achieved to date with DEP. This study comprises of experimental work as well as mathematical modeling using COMSOL. In particular, we establish the parameters in a capillary-based microfluidic system giving rise to these optimum cellcell contact configurations, together with the possibility for facilitating other cell manipulations such as spinning and rotation, thus providing useful protocols for application into microfluidic bioparticle manipulation systems for diagnostics, therapeutics or for furthering research in cellular bioelectricity and intercellular interactions.
Subject Chemical Engineering not elsewhere classified
Keyword(s) Dielectrophoresis
Cell contact
Cell chain
Spinning
Rotation
Microfluidics
DOI - identifier 10.1007/s10544-018-0341-1
Copyright notice © Springer Science+Business Media, LLC, part of Springer Nature 2018
ISSN 1387-2176
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