Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystal

Kirian, R, White, T, Holton, J, Chapman, H and Martin, A., et al., 2011, 'Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystal', Acta Crystallographica Section A, vol. 67, no. 2, pp. 131-140.


Document type: Journal Article
Collection: Journal Articles

Title Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystal
Author(s) Kirian, R
White, T
Holton, J
Chapman, H
Martin, A., et al.,
Year 2011
Journal name Acta Crystallographica Section A
Volume number 67
Issue number 2
Start page 131
End page 140
Total pages 10
Publisher Wiley-Blackwell Publishing
Abstract A complete set of structure factors has been extracted from hundreds of thousands of femtosecond single-shot X-ray microdiffraction patterns taken from randomly oriented nanocrystals. The method of Monte Carlo integration over crystallite size and orientation was applied to experimental data from Photosystem I nanocrystals. This arrives at structure factors from many partial reflections without prior knowledge of the particle-size distribution. The data were collected at the Linac Coherent Light Source (the first hard-X-ray laser user facility), to which was fitted a hydrated protein nanocrystal injector jet, according to the method of serial crystallography. The data are single `still' diffraction snapshots, each from a different nanocrystal with sizes ranging between 100 nm and 2 μm, so the angular width of Bragg peaks was dominated by crystal-size effects. These results were compared with single-crystal data recorded from large crystals of Photosystem I at the Advanced Light Source and the quality of the data was found to be similar. The implications for improving the efficiency of data collection by allowing the use of very small crystals, for radiation-damage reduction and for time-resolved diffraction studies at room temperature are discussed.
Subject Condensed Matter Imaging
Keyword(s) nanocrystals
femtosecond diffraction
free-electron lasers
Monte Carlo methods
protein microdiffraction
DOI - identifier 10.1107/S0108767310050981
Copyright notice © International Union of Crystallography
ISSN 2053-2733
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