Complexation of plasmid DNA and poly(ethylene oxide)/poly(propylene oxide) polymers for safe gene delivery

Daima, H, Shankar, S, Gammilonghi, A, Periasamy, S, Bhargava, S and Bansal, V 2018, 'Complexation of plasmid DNA and poly(ethylene oxide)/poly(propylene oxide) polymers for safe gene delivery', Environmental Chemistry Letters, vol. 16, no. 4, pp. 1457-1462.


Document type: Journal Article
Collection: Journal Articles

Title Complexation of plasmid DNA and poly(ethylene oxide)/poly(propylene oxide) polymers for safe gene delivery
Author(s) Daima, H
Shankar, S
Gammilonghi, A
Periasamy, S
Bhargava, S
Bansal, V
Year 2018
Journal name Environmental Chemistry Letters
Volume number 16
Issue number 4
Start page 1457
End page 1462
Total pages 6
Publisher Springer
Abstract Gene delivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells. Gene therapy utilizes gene delivery to deliver genetic material with the goal of treating a disease or condition in the cell. Actual viral vectors may have side effects, while actual systems using metal nanoparticles for gene delivery are toxic. Therefore, we designed here a biocompatible tri-block copolymer PEO20-PPO69-PEO20as a gene delivery vector [PEO: poly(ethylene oxide); PPO: poly(propylene oxide)]. We studied the conjugation of PEO20-PPO69-PEO20and DNA using various techniques. Results of gel retardation assay along with zeta potential and dynamic light scattering provide evidence of DNA sequestration. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy show that the PO43-groups of plasmid DNA are primarily involved during nanoconjugate construction. The integrity and functionality of plasmid DNA within the cellular environment is further demonstrated by the expression of green fluorescent protein gene in Escherichia coli. Overall, our findings support the use of block copolymers as delivery systems for mammalian and plant cells.
Subject Colloid and Surface Chemistry
Biologically Active Molecules
Keyword(s) Environmental toxicity
Gene delivery
Nanoconjugates
Physicochemical
Plasmid DNA
Tri-block copolymer
DOI - identifier 10.1007/s10311-018-0756-1
Copyright notice © 2018 Springer International Publishing AG, part of Springer Nature
ISSN 1610-3653
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