A Modular Millifluidic Homeostatic Imaging Plate for Imaging of Larval Zebrafish

Friedrich, T, Douek, A, Vandestadt, C, Wlodkowic, D and Kaslin, J 2019, 'A Modular Millifluidic Homeostatic Imaging Plate for Imaging of Larval Zebrafish', Zebrafish, vol. 16, no. 1, pp. 37-46.

Document type: Journal Article
Collection: Journal Articles

Title A Modular Millifluidic Homeostatic Imaging Plate for Imaging of Larval Zebrafish
Author(s) Friedrich, T
Douek, A
Vandestadt, C
Wlodkowic, D
Kaslin, J
Year 2019
Journal name Zebrafish
Volume number 16
Issue number 1
Start page 37
End page 46
Total pages 10
Publisher Mary Ann Liebert, Inc.
Abstract Zebrafish larvae are suitable in vivo models for toxicological and pharmacological screens due to their transparency, small size, ex utero development, and genetic and physiological similarity to humans. Using modern imaging techniques, cells and tissues can be dynamically visualized over several days in multiple zebrafish larvae. However, precise specimen immobilization and maintenance of homeostatic conditions remain a challenge for longitudinal studies. A highly customizable mounting configuration with inbuilt means of controlling temperature and media flow would therefore be a valuable tool to facilitate long-term imaging of a large number of specimens. Using three-dimensional printing, we have developed a millifluidic, modular homeostatic imaging plate (HIP), which consists of a customizable sample insert and a temperature-controlled incubation chamber that is continuously perfused, providing an ideal environment for long-term experiments where homeostatic conditions are desired. The HIP is cheap to produce, has a standard microtiter well plate format, and can be fitted to most microscopes. We used the device to image dynamic regeneration of spinal cord neurons. The flexibility and adaptability of the HIP facilitate long-term in vivo imaging of many samples, and can be easily adapted to suit a broad range of specimens.
Subject Animal Neurobiology
Biomedical Instrumentation
Cell Neurochemistry
DOI - identifier 10.1089/zeb.2018.1684
Copyright notice © 2019 Mary Ann Liebert, Inc.
ISSN 1545-8547
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