Measuring translational diffusion of 15N-enriched biomolecules in complex solutions with a simplified 1H-15N HMQC-filtered BEST sequence

Yao, S, Meikle, T, Sethi, A, Separovic, F, Babon, J and Keizer, D 2018, 'Measuring translational diffusion of 15N-enriched biomolecules in complex solutions with a simplified 1H-15N HMQC-filtered BEST sequence', European Biophysics Journal, vol. 47, no. 8, pp. 891-902.


Document type: Journal Article
Collection: Journal Articles

Title Measuring translational diffusion of 15N-enriched biomolecules in complex solutions with a simplified 1H-15N HMQC-filtered BEST sequence
Author(s) Yao, S
Meikle, T
Sethi, A
Separovic, F
Babon, J
Keizer, D
Year 2018
Journal name European Biophysics Journal
Volume number 47
Issue number 8
Start page 891
End page 902
Total pages 12
Publisher Springer
Abstract Pulsed-field gradient nuclear magnetic resonance has seen an increase in applications spanning a broad range of disciplines where molecular translational diffusion properties are of interest. The current study introduces and experimentally evaluates the measurement of translational diffusion coefficients of 15N-enriched biomolecules using a 1H-15N HMQC-filtered band-selective excitation short transient (BEST) sequence as an alternative to the previously described SOFAST-XSTE sequence. The results demonstrate that accurate translational diffusion coefficients of 15N-labelled peptides and proteins can be obtained using this alternative 1H-15N HMQC-filtered BEST sequence which is implementable on NMR spectrometers equipped with probes fitted with a single-axis field gradient, including most cryoprobes dedicated to bio-NMR. The sequence is of potential use for direct quantification of protein or peptide translational diffusion within complex systems, such as in mixtures of macromolecules, crowded solutions, membrane-mimicking media and in bicontinuous cubic phases, where conventional sequences may not be readily applicable due to the presence of intense signals arising from sources other than the protein or peptide under investigation.
Subject Colloid and Surface Chemistry
Physical Chemistry not elsewhere classified
Chemical Sciences not elsewhere classified
Keyword(s) 1H-15N HMQC BEST
Complex solutions
Crowded solutions
Detergent micelles
Isotope-filtered
PFG-NMR
Translational diffusion
DOI - identifier 10.1007/s00249-018-1311-5
Copyright notice © European Biophysical Societies Association 2018
ISSN 0175-7571
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 3 Abstract Views  -  Detailed Statistics
Created: Mon, 29 Apr 2019, 13:04:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us