Study of inhibitory effect of mercury(ii) ion on exonuclease iiivia gel electrophoresis and microfluidic electrophoresis

Yuan, M, Zhu, Y, Lou, X, Chen, C, Wei, G, Lan, M and Zhao, J 2012, 'Study of inhibitory effect of mercury(ii) ion on exonuclease iiivia gel electrophoresis and microfluidic electrophoresis', Analytical Methods, vol. 4, no. 9, pp. 2846-2851.


Document type: Journal Article
Collection: Journal Articles

Title Study of inhibitory effect of mercury(ii) ion on exonuclease iiivia gel electrophoresis and microfluidic electrophoresis
Author(s) Yuan, M
Zhu, Y
Lou, X
Chen, C
Wei, G
Lan, M
Zhao, J
Year 2012
Journal name Analytical Methods
Volume number 4
Issue number 9
Start page 2846
End page 2851
Total pages 6
Publisher Royal Society of Chemistry
Abstract The effect of mercury(ii) ion on the exonucleolytic activity of Exo III was investigated by using microchip-based microfluidic electrophoresis coupled with polyacrylamide gel electrophoresis. This is the first report of this inhibitory effect with qualitative and quantitative analysis. With a series concentration of Exo III, the inhibition was quantitatively examined at various concentrations of mercuric ion ranging from 0 to 1 μM. For a given concentration of Exo III, the inhibition increased with increasing concentration of mercuric ions, whereas increasing concentration of Exo III hindered the inhibition activity. The inhibiting effect of 1 μM Hg < sup > 2+ < /sup > dropped significantly from (84.7 ± 1.95)% to (3.9 ± 1.25)% when the concentration of Exo III was increased from 0.02 to 0.5 units per μL. This study expands the application of microfluidic analytical methods to biochemistry and lays the foundation for the development of environmental research and genetic studies.
Subject Analytical Chemistry not elsewhere classified
Keyword(s) Escherichia-Coli
Mass-Spectrometry
DNA-Damage
Separation
Digestion
Nuclease
Proteins
Cofactor
Enzymes
Probes
DOI - identifier 10.1039/c2ay25214g
Copyright notice This journal is © The Royal Society of Chemistry 2012
ISSN 1759-9660
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