Sensitive label-free oligonucleotide-based microfluidic detection of mercury (II) ion by using exonuclease I

Yuan, M, Zhu, Y, Lou, X, Chen, C, Wei, G, Lan, M and Zhao, J 2012, 'Sensitive label-free oligonucleotide-based microfluidic detection of mercury (II) ion by using exonuclease I', Biosensors and Bioelectronics, vol. 31, no. 1, pp. 330-336.


Document type: Journal Article
Collection: Journal Articles

Title Sensitive label-free oligonucleotide-based microfluidic detection of mercury (II) ion by using exonuclease I
Author(s) Yuan, M
Zhu, Y
Lou, X
Chen, C
Wei, G
Lan, M
Zhao, J
Year 2012
Journal name Biosensors and Bioelectronics
Volume number 31
Issue number 1
Start page 330
End page 336
Total pages 7
Publisher Elsevier
Abstract Mercury is a highly toxic metal that can cause significant harm to humans and aquatic ecosystems. This paper describes a novel approach for mercury (Hg 2+ ) ion detection by using label-free oligonucleotide probes and Escherichia coli exonuclease I (Exo I) in a microfluidic electrophoretic separated platform. Two single-stranded DNAs (ssDNA) TT-21 and TT-44 with 7 Thymine-Thymine mispairs are employed to capture mercury ions. Due to the coordination structure of T-Hg 2+ -T, these ssDNAs are folded into hairpin-like double-stranded DNAs (dsDNA) which are more difficult to be digested by Exo I, as confirmed by polyacrylamide gel electrophoresis (PAGE) analysis. A series of microfluidic capillary electrophoretic separation studies are carried out to investigate the effect of Exo I and mercury ion concentrations on the detected fluorescence intensity. This method has demonstrated a high sensitivity of mercury ion detection with the limit of detection around 15nM or 3ppb. An excellent selectivity of the probe for mercury ions over five interference ions Fe 3+ , Cd 2+ , Pb 2+ , Cu 2+ and Ca 2+ is also revealed. This method could potentially be used for mercury ion detection with high sensitivity and reliability.
Subject Medical Biotechnology not elsewhere classified
Keyword(s) Exonuclease I
Label-free DNA
Mercury ions
Microfluidic detection
DOI - identifier 10.1016/j.bios.2011.10.043
Copyright notice © 2011 Elsevier B.V. All rights reserved.
ISSN 0956-5663
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