RNASeq Analysis of Aedes albopictus Mosquito Midguts after Chikungunya Virus Infection

Vedururu, R, Neave, M, Tachedjian, M, Klein, M, Gorry, P, Duchemin, J and Paradkar, P 2019, 'RNASeq Analysis of Aedes albopictus Mosquito Midguts after Chikungunya Virus Infection', Viruses, vol. 11, no. 6, 513, pp. 1-12.

Document type: Journal Article
Collection: Journal Articles

Title RNASeq Analysis of Aedes albopictus Mosquito Midguts after Chikungunya Virus Infection
Author(s) Vedururu, R
Neave, M
Tachedjian, M
Klein, M
Gorry, P
Duchemin, J
Paradkar, P
Year 2019
Journal name Viruses
Volume number 11
Issue number 6
Article Number 513
Start page 1
End page 12
Total pages 12
Publisher MDPI AG
Abstract Chikungunya virus (CHIKV) is an emerging pathogen around the world and causes significant morbidity in patients. A single amino acid mutation in the envelope protein of CHIKV has led to a shift in vector preference towards Aedes albopictus. While mosquitoes are known to mount an antiviral immune response post-infection, molecular interactions during the course of infection at the tissue level remain largely uncharacterised. We performed whole transcriptome analysis on dissected midguts of Aedes albopictus infected with CHIKV to identify differentially expressed genes. For this, RNA was extracted at two days post-infection (2-dpi) from pooled midguts. We initially identified 25 differentially expressed genes (p-value < 0.05) when mapped to a reference transcriptome. Further, multiple differentially expressed genes were identified from a custom de novo transcriptome, which was assembled using the reads that did not align with the reference genome. Thirteen of the identified transcripts, possibly involved in immunity, were validated by qRT-PCR. Homologues of seven of these genes were also found to be significantly upregulated in Aedes aegypti midguts 2 dpi, indicating a conserved mechanism at play. These results will help us to characterise the molecular interaction between Aedes albopictus and CHIKV and can be utilised to reduce the impact of this viral infection.
Subject Virology
Keyword(s) Chikungunya
Aedes albopictus
Host-pathogen interactions
DOI - identifier 10.3390/v11060513
Copyright notice © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
ISSN 1999-4915
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 15 Abstract Views  -  Detailed Statistics
Created: Fri, 05 Jul 2019, 12:33:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us