A sustainable biomineralization approach for synthesis of highly fluorescent ultra-small Pt nanoclusters

Balu, R, Knott, R, Elvin, C, Hill, A, Choudhury, N and Dutta, N 2019, 'A sustainable biomineralization approach for synthesis of highly fluorescent ultra-small Pt nanoclusters', Biosensors, vol. 9, no. 4, pp. 1-13.


Document type: Journal Article
Collection: Journal Articles

Title A sustainable biomineralization approach for synthesis of highly fluorescent ultra-small Pt nanoclusters
Author(s) Balu, R
Knott, R
Elvin, C
Hill, A
Choudhury, N
Dutta, N
Year 2019
Journal name Biosensors
Volume number 9
Issue number 4
Start page 1
End page 13
Total pages 13
Publisher MDPIAG
Abstract Herein we report the first example of a facile biomineralization process to produce ultra-small-sized highly fluorescent aqueous dispersions of platinum noble metal quantum clusters (Pt-NMQCs) using a multi-stimulus responsive, biomimetic intrinsically disordered protein (IDP), Rec1-resilin. We demonstrate that Rec1-resilin acts concurrently as the host, reducing agent, and stabilizer of the blue-green fluorescent Pt-NMQCs once they are being formed. The photophysical properties, quantum yield, and fluorescence lifetime measurements of the synthesized Pt-NMQCs were examined using UV-Vis and fluorescence spectroscopy. The oxidation state of the Pt-NMQCs was quantitatively analyzed using X-ray photoelectron spectroscopy. Both a small angle X-ray scattering technique and a modeling approach have been attempted to present a detailed understanding of the structure and conformational dynamics of Rec1-resilin as an IDP during the formation of the Pt-NMQCs. It has been demonstrated that the green fluorescent Pt-NMQCs exhibit a high quantum yield of ~7.0% and a lifetime of ~9.5 ns in aqueous media. The change in photoluminescence properties due to the inter-dot interactions between proximal dots and aggregation of the Pt-NMQCs by evaporation was also measured spectroscopically and discussed.
Subject Clinical Chemistry (diagnostics)
Synthesis of Materials
Nanomaterials
Keyword(s) quantum dot
noble metal clusters
fluorescent platinum nanoclusters
biosensors
biomineralization
intrinsically disordered protein
protein polymer
small angle X-ray scattering
DOI - identifier 10.3390/bios9040128
Copyright notice © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
ISSN 2079-6374
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