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Rolling-circle amplification for the detection of active porcine circovirus type 2 DNA replication in vitro Navidad, P, Li, H, Mankertz, A and Meehan, B 2008, 'Rolling-circle amplification for the detection of active porcine circovirus type 2 DNA replication in vitro', Journal of Virological Methods, vol. 152, no. 12, pp. 112-116. Document type: Journal Article Collection: Journal Articles Title Rolling-circle amplification for the detection of active porcine circovirus type 2 DNA replication in vitro Author(s) Navidad, P Li, H Mankertz, A Meehan, B Year 2008 Journal name Journal of Virological Methods Volume number 152 Issue number 12 Start page 112 End page 116 Total pages 5 Publisher Elsevier Abstract Porcine circovirus type 2 (PCV2) infections in pigs have diverse clinical presentations and are considered economically important diseases worldwide. However, despite intensive research, the early pathogenesis of PCV2 and the primary target cells for PCV2 infections and replication are still unknown. Rolling-circle amplification (RCA) is an amplification technique for small, circular DNA templates that essentially mimics rolling-circle replication in vitro. In this study, the amplification of PCV2-specific DNAs using randomly primed RCA has been demonstrated. This novel approach has circumvented the normal requirement for conventional virus isolation procedures for the characterization of PCV2 DNAs from clinical samples. In addition, the potential utility of a strand-specific derivative of RCA was further investigated. Specifically, strand-specific RCA for the detection of active virus replication following the amplification of complementary sense PCV2 DNAs, which occur as double-stranded replicative intermediates that are present only during de novo viral DNA replication both in vitro and in vivo has been demonstrated. Subject Microbiology not elsewhere classified Keyword(s) Rolling-circle amplification (RCA) Rolling-circle replication (RCR) Porcine circovirus diseases (PCVDs) Porcine circovirus type 2 (PCV2) Postweaning multisystemic wasting syndrome (PMWS) DOI - identifier 10.1016/j.jviromet.2008.05.026 ISSN 0166-0934 Related Links Link Description http://dx.doi.org/10.1016/j.jviromet.2008.05.026 Link to Published Version   Versions Version Filter Type Mon, 06 Dec 2010, 14:15:25 EST Tue, 17 Jun 2014, 23:46:55 EST Filtered Full Citation counts:   Cited 5 times in Thomson Reuters Web of Science Article | Citations Cited 4 times in Scopus Article | Citations Altmetric details: Access Statistics: 261 Abstract Views  -  Detailed Statistics Created: Mon, 06 Dec 2010, 11:15:00 EST by Catalyst Administrator