Isolating conifer DNA: A superior polysaccharide elimination method

Crowley, T, Muralitharan, M and Stevenson, T 2003, 'Isolating conifer DNA: A superior polysaccharide elimination method', Plant Molecular Biology Reporter, vol. 21, no. 1, pp. 97a-97d.


Document type: Journal Article
Collection: Journal Articles

Title Isolating conifer DNA: A superior polysaccharide elimination method
Author(s) Crowley, T
Muralitharan, M
Stevenson, T
Year 2003
Journal name Plant Molecular Biology Reporter
Volume number 21
Issue number 1
Start page 97a
End page 97d
Total pages 1
Publisher International Society for Plant Molecular Biology
Abstract Genomic DNA was isolated from frozen needles of mature Pinus radiata clones using a modified extraction technique incorporating cetyltrimethylammonium bromide (CTAB) for cell lysis. A high sodium chloride concentration (2 M) was used at 2 stages of the extraction procedure to eradicate polysaccharides, yielding pure genomic DNA suitable for restriction enzyme digestion and PCR amplification. Extractions were scaled down to suit 1.5-mL Eppendorf tubes, allowing easier handling and enhanced sterility.
Subject Plant Cell and Molecular Biology
ISSN 0735-9640
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