Identification of a sequence from the genome of porcine circovirus type 2 with an inhibitory effect on IFN-alpha production by porcine PBMCs

Hasslung, F, Berg, M, Allan, G, Meehan, B, McNeilly, F and Fossum, C 2003, 'Identification of a sequence from the genome of porcine circovirus type 2 with an inhibitory effect on IFN-alpha production by porcine PBMCs', Journal of General Virology, vol. 84, no. 11, pp. 2937-2945.


Document type: Journal Article
Collection: Journal Articles

Title Identification of a sequence from the genome of porcine circovirus type 2 with an inhibitory effect on IFN-alpha production by porcine PBMCs
Author(s) Hasslung, F
Berg, M
Allan, G
Meehan, B
McNeilly, F
Fossum, C
Year 2003
Journal name Journal of General Virology
Volume number 84
Issue number 11
Start page 2937
End page 2945
Total pages 9
Publisher Society for General Microbiology
Abstract Porcine circovirus type 2 (PCV-2) has been identified as the causal agent of postweaning multisystemic wasting syndrome and has been associated with several other disease syndromes in pigs. To date, however, little is known regarding the mechanism(s) underlying the pathogenesis of PCV-2-induced diseases and the interaction of the virus with the host immune system. In the present study, oligodeoxynucleotides (ODNs), with central CpG motifs selected from the genome of PCV-2, were demonstrated to modulate the immune response of porcine PBMCs. Four of the five ODNs tested were demonstrated to act in a stimulatory manner via induction of IFN-? production, whereas only one of the five ODNs showed inhibitory activity. Also, this inhibitory ODN was demonstrated to completely inhibit IFN-? production induced by the other stimulatory ODNs and showed a variable degree of inhibitory action on other known inducers of IFN-?. Although no single common characteristic among resistant or susceptible inducers could be identified, the presence of immune modulatory sequences in the genome of PCV-2 may represent an underlying mechanism of the pathogenesis of PCV-2-associated diseases.
Subject Medical Microbiology not elsewhere classified
Keyword(s) animal cell
article
Circoviridae
controlled study
CpG island
disease association
gene sequence
host resistance
immune response
immunomodulation
immunostimulation
interferon production
nonhuman
nucleotide sequence
peripheral lymphocyte
priority journal
protein motif
sequence analysis
swine
virus cell interaction
virus genome
virus pathogenesis
wasting syndrome
weaning
DOI - identifier 10.1099/vir.0.19362-0
ISSN 0022-1317
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