Hierarchy of DNA immobilization and hybridization on poly-L-lysine: An atomic force microscopy study

Sawant, P and Nicolau, D 2006, 'Hierarchy of DNA immobilization and hybridization on poly-L-lysine: An atomic force microscopy study', Smart Materials and Structures, vol. 15, no. 1, pp. S99-S103.


Document type: Journal Article
Collection: Journal Articles

Title Hierarchy of DNA immobilization and hybridization on poly-L-lysine: An atomic force microscopy study
Author(s) Sawant, P
Nicolau, D
Year 2006
Journal name Smart Materials and Structures
Volume number 15
Issue number 1
Start page S99
End page S103
Total pages 4
Publisher Institute of Physics Publishing Ltd.
Abstract Atomic force microscopy is used to analyze the immobilization of single-stranded DNA (ssDNA) on poly-L-lysine (PL)-coated glass and subsequent hybridization with complementary DNA (dsDNA). 2D fractal analysis (power spectrum density algorithm (PSDA)) and line analysis methods are used to characterize the vertical aggregation of ssDNA/dsDNA. The nanodefects on the PL layer could be responsible for the vertical aggregation of DNA. The line analysis suggests that approximately two layers of ssDNA are adsorbed, stacked vertically. The PSDA is useful in understanding the direction of aggregation in addition to understanding the growth mechanism. Fractal dimension values are between 2 and 3, suggesting that the growth mechanism may obey the diffusion-limited aggregation (DLA) model.
Subject Materials Engineering not elsewhere classified
DOI - identifier 10.1088/0964-1726/15/1/016
ISSN 0964-1726
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 5 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 68 Abstract Views  -  Detailed Statistics
Created: Mon, 06 Dec 2010, 14:11:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us