Hierarchy of DNA immobilization and hybridization on poly-L-lysine using an atomic force microscopy study

Sawant, P, Watson, G, Nicolau, D, Myhra, S and Nicolau, D 2005, 'Hierarchy of DNA immobilization and hybridization on poly-L-lysine using an atomic force microscopy study', Journal of Nanoscience and Nanotechnology, vol. 5, no. 6, pp. 951-957.


Document type: Journal Article
Collection: Journal Articles

Title Hierarchy of DNA immobilization and hybridization on poly-L-lysine using an atomic force microscopy study
Author(s) Sawant, P
Watson, G
Nicolau, D
Myhra, S
Nicolau, D
Year 2005
Journal name Journal of Nanoscience and Nanotechnology
Volume number 5
Issue number 6
Start page 951
End page 957
Total pages 7
Publisher American Scientific Publishers
Abstract The atomic force microscopy has been used to analyze the immobilization of single stranded DNA on poly-L-lysine-coated glass and subsequent hybridization with complimentary DNA with the Z-threshold parameter and fractal analysis methods. The poly-L-lysine layer, which has a thickness of approximately 7 nm, presents nano-defects that could be critical for DNA immobilization by acting as a nucleation sites for ssDNA and subsequently for dsDNA aggregates. The Z-threshold for the dsDNA aggregates is much larger than for ssDNA, but the statistical fractal dimension is very similar, suggesting a conformal increase of the dimensions of the dsDNA aggregates mainly in the Z-direction, due to an effective ssDNA-ccDNA molecular recognition. This study demonstrates the use of fractal analysis in conjunction with the distribution of heights to evaluate the efficiency of DNA-DNA molecular recognition on surfaces and the impact of nanodefects.
Subject Nanobiotechnology
Keyword(s) AFM
Biochips
DNA
Hybridization
Immobilization
Nanodefects
Poly-L-lysine
DOI - identifier 10.1166/jnn.2005.125
Copyright notice Copyright © 2005 American Scientific Publishers All rights reserved
ISSN 1533-4880
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 89 Abstract Views  -  Detailed Statistics
Created: Mon, 06 Dec 2010, 14:11:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us