So you need a protein - A guide to the production of recombinant proteins

Jayaraj, R and Smooker, P 2009, 'So you need a protein - A guide to the production of recombinant proteins', The Open Veterinary Science Journal, vol. 3, no. 7, pp. 28-34.


Document type: Journal Article
Collection: Journal Articles

Title So you need a protein - A guide to the production of recombinant proteins
Author(s) Jayaraj, R
Smooker, P
Year 2009
Journal name The Open Veterinary Science Journal
Volume number 3
Issue number 7
Start page 28
End page 34
Total pages 7
Publisher Bentham Open
Abstract The field of biotechnology owes a great deal to the ability to produce recombinant proteins, which can be made in far greater abundance than many native proteins, and are more easily quality controlled. There is a great need for individual proteins to be produced for research purposes. This review is aimed at researchers who are not experienced at protein expression, but find that they have a need to produce a recombinant protein. We detail the major expression systems that will be commonly used in the laboratory situation- bacterial, yeast and insect cell culture. The application of each, and the relative advantages/disadvantages are discussed.
Subject Industrial Molecular Engineering of Nucleic Acids and Proteins
Keyword(s) biotechnology
recombinant proteins
native proteins
protein expression
bacteria culture
yeast culture
insect cell culture
DOI - identifier 10.2174/1874318800903010028
ISSN 1874-3188
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