Design and application of a peptide nucleic acid sequence targeting the p75 neurotrophin receptor

Cheah, I, Cheema, S, Langford, S, Lopes, E, KJ,, Petratos, S and Turner, B 2003, 'Design and application of a peptide nucleic acid sequence targeting the p75 neurotrophin receptor', Bioorganic and Medicinal Chemistry Letters, vol. 13, no. 14, pp. 2377-2380.


Document type: Journal Article
Collection: Journal Articles

Title Design and application of a peptide nucleic acid sequence targeting the p75 neurotrophin receptor
Author(s) Cheah, I
Cheema, S
Langford, S
Lopes, E
KJ,
Petratos, S
Turner, B
Year 2003
Journal name Bioorganic and Medicinal Chemistry Letters
Volume number 13
Issue number 14
Start page 2377
End page 2380
Total pages 4
Publisher Pergamon
Abstract Novel antisense peptide nucleic acid (PNA) constructs targeting p75NTR as a potential therapeutic strategy for amyotrophic lateral sclerosis (ALS) were designed, synthesised and evaluated against phosphorothioate oligonucleotide sequences (PS-ODN). An 11-mer antisense PNA directed at the initiation codon dose-dependently inhibited p75NTR expression and death signalling by nerve growth factor in Schwann cell cultures. Inhibition of p75NTR production was not detected in cultures treated with the nonsense PNA or antisense PNA directed at the 3'-terminus sequence. The 19-mer PS-ODN sequences also failed to confer any activity against p75NTR but, unlike the PNA sequences, were toxic in vitro at comparable doses.
Subject Pharmacology and Pharmaceutical Sciences not elsewhere classified
Keyword(s) cell death
down-regulation
drug design
indicators and reagents
molecular weight
motor neuron disease
nerve growth factor
neurodegenerative diseases
oligonucleotides
antisense
peptides
receptor
nerve growth factor
receptors
nerve growth factor
Schwann cells
signal transduction
DOI - identifier 10.1016/S0960-894X(03)00400-1
Copyright notice © 2003 Elsevier Science Ltd. All rights reserved.
ISSN 0960-894X
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 4 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 104 Abstract Views  -  Detailed Statistics
Created: Mon, 06 Dec 2010, 14:11:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us