Nano-topographic evaluation of highly disordered fractal-like structures of immobilized oligonucleotides using AFM

Sawant, P and Nicolau, D 2006, 'Nano-topographic evaluation of highly disordered fractal-like structures of immobilized oligonucleotides using AFM', Materials Science and Engineering B: Solid-State Materials for Advanced Technology, vol. 132, no. 39845, pp. 147-150.


Document type: Journal Article
Collection: Journal Articles

Title Nano-topographic evaluation of highly disordered fractal-like structures of immobilized oligonucleotides using AFM
Author(s) Sawant, P
Nicolau, D
Year 2006
Journal name Materials Science and Engineering B: Solid-State Materials for Advanced Technology
Volume number 132
Issue number 39845
Start page 147
End page 150
Total pages 4
Publisher Elsevier BV
Abstract In a recent trend of micro- and nano-array technologies, polymers are gaining preference over traditional substrates such as glass, silicates, etc. as a model biosurface to immobilize biomolecules. In present paper, we compared model polymeric surfaces such as cyclo olefin copolymer (COC) and polycarbonate (PC), with traditional surfaces such as silicone-wafer and mica. We used a 2D fractal dimension method, i.e. the perimeter-area relationship (PAR) to study the immobilization of 26 base pair oligonucleotide primer on surfaces which are imaged by AFM. Results revealed that the efficiency of the vertical immobilization is in the following order: COC > PC > mica > Si-wafer which can be contributed to chemical and nano-topographical heterogeneity. This study is useful for in-depth understanding of fundamental issues such as effects of manufacturing parameters and evaluation of surface nanotopographies for the development of high-density biochips.
Subject Colloid and Surface Chemistry
Keyword(s) 2D-fractal dimension
Biochip
Bionanotechnology
Nanotopography
Oligonucleotides
Polymers
DOI - identifier 10.1016/j.mseb.2006.02.044
Copyright notice © 2006 Elsevier B.V. All rights reserved
ISSN 0921-5107
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