3',4'-Dihydroxyflavonol enhances nitric oxide bioavailability and improves vascular function after ischaemia and reperfusion injury in the rat.

Chan, E, Drummond, G and Woodman, O 2003, '3',4'-Dihydroxyflavonol enhances nitric oxide bioavailability and improves vascular function after ischaemia and reperfusion injury in the rat.', Journal of Cardiovascular Pharmacology, vol. 42, no. 6, pp. 727-735.


Document type: Journal Article
Collection: Journal Articles

Title 3',4'-Dihydroxyflavonol enhances nitric oxide bioavailability and improves vascular function after ischaemia and reperfusion injury in the rat.
Author(s) Chan, E
Drummond, G
Woodman, O
Year 2003
Journal name Journal of Cardiovascular Pharmacology
Volume number 42
Issue number 6
Start page 727
End page 735
Total pages 9
Publisher Lippincott Williams & Wilkins
Abstract We hypothesized that 3',4'-dihydroxyflavonol (DiOHF) by scavenging superoxide anions (O2-.) would increase the bioavailability of NO and potentiate NO-mediated relaxation in the rat aorta. Furthermore we hypothesized that DiOHF, by its antioxidant activity, would preserve responses to acetylcholine (ACh) in the presence of O2-. generators in the aorta in vitro and after ischemia and reperfusion of the rat hindquarters vasculature in situ. Using lucigenin-enhanced chemiluminescence we demonstrated that DiOHF caused a concentration-dependent reduction in O2-. accumulation whether generated by xanthine/xanthine oxidase in a cell-free system or by rat isolated aorta in the presence of NADPH. DiOHF also prevented the inhibitory effects of xanthine/xanthine oxidase and pyrogallol on vasorelaxation to ACh and sodium nitroprusside (SNP) in the rat aorta in vitro, and attenuated the vascular dysfunction caused by 2 h ischemia and 2 h reperfusion (I/R) in the rat hindquarters. I/R significantly reduced the dilator responses to both ACh and SNP; however, this effect was attenuated when DiOHF was given before the onset of ischemia or reperfusion. In conclusion, DiOHF, by scavenging O 2-., increases the relaxant activity of ACh and SNP and reduces the degree of inhibition of xanthine/xanthine oxidase or pyrogallol on the response to ACh. DiOHF reduces the adverse effects of I/R on vascular function by increasing NO bioavailability suggesting that it may be useful in preventing reperfusion injury.
Subject Basic Pharmacology
Keyword(s) flavonol
ischemia
nitric oxide
oxygen radicals
reperfusion
DOI - identifier 10.1097/00005344-200312000-00006
ISSN 0160-2446
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