Cytoskeletal anchoring of GLAST determines susceptibility to brain damage: An identified role for GFAP

Sullivan, S, Lee, A, Bjorkman, S, Miller, S, Sullivan, R, Poronnik, P, Colditz, P and Pow, D 2007, 'Cytoskeletal anchoring of GLAST determines susceptibility to brain damage: An identified role for GFAP', Journal of Biological Chemistry, vol. 282, no. 40, pp. 29414-29423.


Document type: Journal Article
Collection: Journal Articles

Title Cytoskeletal anchoring of GLAST determines susceptibility to brain damage: An identified role for GFAP
Author(s) Sullivan, S
Lee, A
Bjorkman, S
Miller, S
Sullivan, R
Poronnik, P
Colditz, P
Pow, D
Year 2007
Journal name Journal of Biological Chemistry
Volume number 282
Issue number 40
Start page 29414
End page 29423
Total pages 10
Publisher American Society for Biochemistry and Molecular Biology
Abstract Glial fibrillary acidic protein (GFAP) is an enigmatic protein; it currently has no unambiguously defined role. It is expressed in the cytoskeleton of astrocytes in the mammalian brain. We have used co-immunoprecipitation to identify in vivo binding partners for GFAP in the rat and pig brain. We demonstrate interactions between GFAP, the glutamate transporter GLAST, the PDZ-binding protein NHERF1, and ezrin. These interactions are physiologically relevant; we demonstrate in vitro that transport of D-aspartate (a glutamate analogue) is significantly increased in the presence of GFAP and NHERF1. Moreover, we demonstrate in vivo that expression of GFAP is essential in retaining GLAST in the plasma membranes of astrocytes after an hypoxic insult. These data indicate that the cytoskeleton of the astrocyte plays an important role in protecting the brain against glutamate-mediated excitotoxicity.
Subject Biochemistry and Cell Biology not elsewhere classified
DOI - identifier 10.1074/jbc.M704152200
ISSN 0021-9258
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 71 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 110 Abstract Views  -  Detailed Statistics
Created: Mon, 06 Dec 2010, 14:11:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us