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Simultaneous determination of the lactone and carboxylate forms of irinotecan (CPT-11) and its active metabolite SN-38 by high-performance liquid chromatography: Application to plasma pharmacokinetic studies in the rat Yang, X, Hu, Z, Chan, S, Goh, B, Chan, E, Ho, P and Zhou, S 2005, 'Simultaneous determination of the lactone and carboxylate forms of irinotecan (CPT-11) and its active metabolite SN-38 by high-performance liquid chromatography: Application to plasma pharmacokinetic studies in the rat', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 821, no. 2, pp. 221-228. Document type: Journal Article Collection: Journal Articles Title Simultaneous determination of the lactone and carboxylate forms of irinotecan (CPT-11) and its active metabolite SN-38 by high-performance liquid chromatography: Application to plasma pharmacokinetic studies in the rat Author(s) Yang, X Hu, Z Chan, S Goh, B Chan, E Ho, P Zhou, S Year 2005 Journal name Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences Volume number 821 Issue number 2 Start page 221 End page 228 Total pages 8 Publisher Elsevier BV Abstract Irinotecan (CPT-11) and its main metabolite SN-38 are potent anticancer derivatives of camptothecin (CPT), with active lactone and inactive carboxylate forms coexisting. A simple and sensitive HPLC method using the ion-pairing reagent tetrabutylammonium hydrogen sulfate (TBAHS) was developed to simultaneously determine all four analytes in rat plasma samples. Camptothecin (CPT) was used as internal standard. The mobile phase was 0.1 M potassium dihydrogen phosphate containing 0.01 M TBAHS (pH 6.4)-acetonitrile (75:25, v/v). Separation of the compounds was carried out on a Hypersil C18 column, monitored at 540 nm (excitation wavelength at 380 nm). All four compounds gave linear response as a function of concentration over 0.01-10 μM. The limit of quantitation in rat plasma was 0.01, 0.008, 0.005 and 0.005 μM for CPT-11 lactone, CPT-11 carboxylate, SN-38 lactone and SN-38 carboxylate, respectively. The method was successfully used in the study on the effect of coadministered thalidomide on the plasma pharmacokinetics of CPT-11 and SN-38 in rats. Coadministered thalidomide (100 mg/kg body weight by intraperitoneal injection) significantly increased the AUC0-10h values of CPT-11 lactone and CPT-11 carboxylate by 32.6% and 30.3 %, respectively, (P < 0.01), but decreased the values by 19.2% and 32.4% for SN-38 lactone and carboxylate, respectively, (P < 0.05). Accordingly, the value of total body clearance (CL) of CPT-11 lactone was significantly lower in combination group compared to the control (1.329 versus 1.837 L/h/kg, P = 0.0002). Plasma t1/2ß values for SN-38 lactone and carboxylate were significantly (P < 0.01) smaller in rats with coadministered thalidomide, as compared to rats receiving CPT-11 alone. Further studies are needed to explore the underlying mechanisms for the observed kinetic interaction between CPT-11 and thalidomide. Subject Basic Pharmacology DOI - identifier 10.1016/j.jchromb.2005.05.010 ISSN 1570-0232 Related Links Link Description http://dx.doi.org/10.1016/j.jchromb.2005.05.010 Link to Published Version   Versions Version Filter Type Mon, 06 Dec 2010, 16:10:03 EST Wed, 18 Jun 2014, 00:00:53 EST Filtered Full Citation counts: Cited 43 times in Scopus Article | Citations Altmetric details: Access Statistics: 109 Abstract Views  -  Detailed Statistics Created: Mon, 06 Dec 2010, 14:11:00 EST by Catalyst Administrator