Involvement of the GC-rich sequence and specific proteins (Sp1/Sp3) in the basal transcription activity of neurogranin gene

Gui, J, Song, Y, Hana, n, Zhou, S and Sheu, F 2006, 'Involvement of the GC-rich sequence and specific proteins (Sp1/Sp3) in the basal transcription activity of neurogranin gene', Biochemical and Biophysical Research Communications, vol. 345, no. 1, pp. 124-132.


Document type: Journal Article
Collection: Journal Articles

Title Involvement of the GC-rich sequence and specific proteins (Sp1/Sp3) in the basal transcription activity of neurogranin gene
Author(s) Gui, J
Song, Y
Hana, n
Zhou, S
Sheu, F
Year 2006
Journal name Biochemical and Biophysical Research Communications
Volume number 345
Issue number 1
Start page 124
End page 132
Total pages 9
Publisher Academic Press
Abstract Neurogranin (Ng), a neuronal protein implicated in learning and memory, contains a TATA-less promoter. Analysis of 5'-deletion mutations and site-directed mutations of the mouse Ng promoter revealed that a 258 bp 5'-flanking sequence (+3 to +260) conferred the basal transcription activity, and that the GC-rich sequence (+22 to +33) served as an important determinant of the promoter activity. Transient transfection of the Sp1 expression plasmid transactivated the reporter activity in neuroblastoma N2A cells while knocking down of endogenous Sp1 expression resulted in a 2.5-fold reduction of the reporter activity in HEK 293 cells. Exogenous expression of Sp3 in HEK 293 cells, however, repressed the reporter activity by 50%. Nevertheless, by gel shift assays, Sp1 and Sp3 were not found to be responsible for the protein-DNA complexes formed by the GC-rich sequence. Moreover, a nuclear factor from the mouse brain tissues was discovered to bind to multiple AT-rich regions in Ng promoter.
Subject Biochemistry and Cell Biology not elsewhere classified
DOI - identifier 10.1016/j.bbrc.2006.04.054
ISSN 0006-291X
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