Rapid determination of common mutations in glutathione s-transferase gene by pcr-based methods in healthy chinese

Zhong, S, Zhou, S, Chen, X and Huang, M 2006, 'Rapid determination of common mutations in glutathione s-transferase gene by pcr-based methods in healthy chinese', Clinica Chimica Acta, vol. 364, no. 12, pp. 205-208.


Document type: Journal Article
Collection: Journal Articles

Title Rapid determination of common mutations in glutathione s-transferase gene by pcr-based methods in healthy chinese
Author(s) Zhong, S
Zhou, S
Chen, X
Huang, M
Year 2006
Journal name Clinica Chimica Acta
Volume number 364
Issue number 12
Start page 205
End page 208
Total pages 4
Publisher Elsevier BV
Abstract Background: The glutathione S-transferase (GST) superfamily comprises multiple isozymes with compelling evidence of functional polymorphisms in various ethnic groups. All these mutations, in particular those in class μ, p and ? GST, are likely to contribute to interindividual differences in responses to xenobiotics including response to chemotherapy and associated with altered disease. The frequency of common GST mutations in Uygur Chinese is unknown. We investigated the common mutations of GSTM1, GSTT1, and GSTP1 in Uygur (N = 154) Chinese and compare with Han Chinese (N = 196). Method: GSTM1 and GSTT1 polymorphisms were analyzed by multiplexed PCR, and GSTP1 polymorphism was detected by PCR-based restriction fragment length polymorphism (RFLP) analysis. Results: GSTM1 null genotype was found in 53.2% Uygur Chinese, which was close to that in Han Chinese (56.1%) (P = 0.592). A significantly lower frequency (P < 0.05) of GSTT1 null genotype in Uygur Chinese (26.6%) was observed compared with Han Chinese (50.0%). Uygur Chinese exhibited a GSTP1 genotype distribution of 51.3% I/I, 40.2% I/V and 8.4% V/V, which was different from that in Han Chinese (60.7% I/I, 35.2% I/V and 4.1% V/V). Conclusions: There is marked ethnic difference in the frequency of common GSTT1 and GSTP1 mutation, but not GSTM1 mutation, between Uygur and Han Chinese.
Subject Basic Pharmacology
DOI - identifier 10.1016/j.cccn.2005.06.021
Copyright notice © 2005 Elsevier B.V. All rights reserved.
ISSN 0009-8981
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