Detection of aristolochic acid I, tetrandrine and fangchinoline in medicinal plants by high performance liquid chromatography and liquid chromatography/mass spectrometry

Koh, H, Wang, H, Zhou, S, Chan, E and Woo, S 2006, 'Detection of aristolochic acid I, tetrandrine and fangchinoline in medicinal plants by high performance liquid chromatography and liquid chromatography/mass spectrometry', Journal of Pharmaceutical and Biomedical Analysis, vol. 40, no. 3, pp. 653-661.


Document type: Journal Article
Collection: Journal Articles

Title Detection of aristolochic acid I, tetrandrine and fangchinoline in medicinal plants by high performance liquid chromatography and liquid chromatography/mass spectrometry
Author(s) Koh, H
Wang, H
Zhou, S
Chan, E
Woo, S
Year 2006
Journal name Journal of Pharmaceutical and Biomedical Analysis
Volume number 40
Issue number 3
Start page 653
End page 661
Total pages 9
Publisher Elsevier BV
Abstract Problems with identification and labeling of medicinal plants, as well as substitution/adulteration of non-toxic plants by toxic ones have previously led to cancer, renal failure and even deaths. The non-toxic Stephania tetrandra (Fangji) has been known to be substituted by Aristolochia fangchi (Guang fangji), which contains the nephrotoxic and carcinogenic aristolochic acid (AA). In this study, 10 samples of "Fangji" were bought from local medicinal shops. HPLC-DAD chromatographic fingerprints of each methanol extract were compared with those of A. fangchi and S. tetrandra, using aristolochic acid I (AAI), tetrandrine and fangchinoline as marker compounds. Nine of the samples were found to be similar to A. fangchi. The presence of AAI in the nine samples was confirmed using LC-MS/MS. Neither tetrandrine nor fangchinoline were detected in these samples. The methods developed in this study allow the simultaneous detection of AAI, fangchinoline and tetrandrine. The results suggest possible substitution of S. tetrandra by A. fangchi at wholesale or retail level. This study highlights the importance of greater control of medicinal plants with toxic components as these may still be readily accessible to the public.
Subject Analytical Chemistry not elsewhere classified
DOI - identifier 10.1016/j.jpba.2005.08.001
ISSN 0731-7085
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 37 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 145 Abstract Views  -  Detailed Statistics
Created: Mon, 06 Dec 2010, 14:11:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us