Characterization of a haemolytic phospholipase A2 activity in clinical isolates of Campylobacter concisus

Istivan, T, Coloe, P, Fry, B, Ward, P and Smith, S 2004, 'Characterization of a haemolytic phospholipase A2 activity in clinical isolates of Campylobacter concisus', Journal of Medical Microbiology, vol. 53, no. 6, pp. 483-493.


Document type: Journal Article
Collection: Journal Articles

Title Characterization of a haemolytic phospholipase A2 activity in clinical isolates of Campylobacter concisus
Author(s) Istivan, T
Coloe, P
Fry, B
Ward, P
Smith, S
Year 2004
Journal name Journal of Medical Microbiology
Volume number 53
Issue number 6
Start page 483
End page 493
Total pages 11
Publisher Society for General Microbiology
Abstract A membrane-bound, haemolytic phospholipase A(2) (PLA(2)) activity was detected in clinical strains of Campylobacter concisus isolated from children with gastroenteritis. The clinical strains were assigned into two molecular groups (genomospecies) based on PCR amplification of their 23S rDNA. This calcium-dependent, heat-stable, haemolytic PLA2 activity was detected in strains from both genomospecies. A crude haemolysin extract (CHE) was initially prepared from cellular outermembrane proteins of these isolates and was further fractionated by ultrafiltration. The haemolytic activity of the extracted fraction (R30) was retained by ultrafiltration using a 30 kDa molecular mass cut-off filter, and was designated haemolysin extract (HE). Both CHE and HE had PLA(2) activity and caused stable vacuolating and cytolytic effects on Chinese hamster ovary cells in tissue culture. Primers for the conserved region of p/dA gene (phospholipase A gene) from Campylobacter coli amplified a gene region of 460 bp in all tested isolates, confirming the presence of a homologous PLA gene sequence in C. concisus. The detection of haemolytic PLA(2) activity in C. concisus indicates the presence of a potential virulence factor in this species and supports the hypothesis that C. concisus is a possible opportunistic pathogen.
Subject Medical Microbiology not elsewhere classified
Keyword(s) Haemolytic phospholipase A2
Campylobactor concisus
Campylobactor coli
gastroenteritis
phospholipase A gene
DOI - identifier 10.1099/jmm.0.45554-0
Copyright notice © 2004 Society for General Microbiology
ISSN 0022-2615
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